Catabolite repression in Bacillus subtilis: a global regulatory mechanism for the Gram-positive bacteria?
| Autor: | Wolfgang Hillen, Christoph J. Hueck |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Mutant Catabolite repression macromolecular substances Bacillus subtilis Regulatory Sequences Nucleic Acid Gram-Positive Bacteria Microbiology Serine chemistry.chemical_compound Bacterial Proteins Amino Acid Sequence Phosphorylation Phosphoenolpyruvate Sugar Phosphotransferase System Molecular Biology Bacillus megaterium Base Sequence biology PEP group translocation biology.organism_classification DNA-Binding Proteins Repressor Proteins carbohydrates (lipids) Gene Expression Regulation Biochemistry chemistry Regulatory sequence Amylases CCPA bacteria Protein Processing Post-Translational Signal Transduction |
| Zdroj: | Molecular Microbiology. 15:395-401 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1995.tb02252.x |
| Popis: | Three components involved in catabolite repression (CR) of gene expression in Bacillus have been identified. The cis-acting catabolite responsive element (CRE), which is present in many genes encoding carbon catabolic enzymes in various species of the Gram-positive bacteria, mediates CR of several genes in Bacillus subtilis, Bacillus megaterium, and Staphylococcus xylosus. CR of most genes regulated via CRE is also affected by the trans-acting factors CcpA and HPr. Similarities between CcpA and Lac and Gal repressors suggest binding of CcpA to CRE. HPr, a component of the phosphoenolpyruvate:sugar phosphotransferase system, undergoes regulatory phosphorylation at a serine residue by a fructose-1,6-diphosphate-activated kinase. A mutant of HPr, which is not phosphorylatable at this position because of an exchange of serine to alanine, lacks CR of several catabolic activities. This mutant phenotype is similar to the one exhibited by a ccpA mutant. Direct protein-protein interaction between CcpA and HPr(Ser-P) was recently demonstrated and constitutes a link between metabolic activity and CR. |
| Databáze: | OpenAIRE |
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