Precise location of two novel linear epitopes on the receptor-binding domain surface of MERS-CoV spike protein recognized by two different monoclonal antibodies
| Autor: | Pan, Wang, Peiyang, Ding, Qiang, Wei, Hongliang, Liu, Yunchao, Liu, Qingmei, Li, Yunrui, Xing, Ge, Li, Enmin, Zhou, Gaiping, Zhang |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Monoclonal antibody
Mice Inbred BALB C Novel linear B cell epitopes General Medicine Spodoptera Antibodies Viral Biochemistry Article Antibodies Monoclonal Murine-Derived Epitopes Mice HEK293 Cells Protein Domains MERS-CoV RBD Structural Biology Spike Glycoprotein Coronavirus Middle East Respiratory Syndrome Coronavirus Sf9 Cells Animals Humans Molecular Biology Epitope Mapping |
| Zdroj: | International Journal of Biological Macromolecules |
| ISSN: | 0141-8130 |
| Popis: | Middle East respiratory syndrome coronavirus (MERS-CoV) is a coronavirus which can cause severe human respiratory diseases with a fatality rate of almost 36%. In this study, we report the generation, characterization and epitope mapping of several monoclonal antibodies against the spike receptor-binding domain (RBD) of MERS-CoV. Two monoclonal antibodies (4C7 and 6E8) that can react with linearized RBD have been selected for subsequent identification of RBD mAb-binding epitopes. Two distinct novel linear epitopes, 423FTCSQIS429 and 546SPLEGGGWL554,were precisely located at the outermost surface of RBD by dot-blot hybridization and ELISAs. Multiple sequence alignment analysis showed that these two peptides were highly conserved. Alanine (A)-scanning mutagenesis demonstrated that residues 423F, 428I, and 429S are the crucial residues for the linear epitope 423FTCSQIS429 while residues 548L, 550G, 553W, 554L for epitope 546SPLEGGGWL554. These findings may be helpful for further understanding of the function of RBD protein and the development of subsequent diagnosis and detection methods. |
| Databáze: | OpenAIRE |
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