The interaction between RACK1 and WEE1 regulates the growth of gastric cancer cell line HGC27
| Autor: | Chao Liu, Yizhao Wang, Li-Li Ren, Jianying Xiao, Yi-Meng Liu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
RACK1 Cancer Research Oncogene gastric cancer HGC27 Cell Articles Cell cycle Biology medicine.disease_cause 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology Downregulation and upregulation Apoptosis 030220 oncology & carcinogenesis medicine Cancer research WEE1 Signal transduction Carcinogenesis A431 cells |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | Receptor of activated C Kinase 1 (RACK1) is an essential scaffold and anchoring protein, which serves an important role in multiple tumorigenesis signaling pathways. The present study aimed to investigate the expression of RACK1 in gastric cancer (GC), and its association with the occurrence and development of GC. In addition, the effect and mechanism of RACK1 overexpression on the growth, and proliferation of GC cells was examined. Firstly, the protein expression of RACK1 was detected in 70 cases of GC tissues and 30 cases of noncancerous tissues using immunohistochemical staining, and the association between clinical and pathological features of GC was analyzed. Secondly, the mRNA and protein expression of RACK1 was determined in the poorly-differentiated human gastric cancer cell line HGC27 and gastric epithelial cell line GES-1. The growth of HGC27 cells following the upregulation of RACK1 was detected using MTT method. Subsequently, the interaction and co-location between RACK1, and WEE1 homolog (S. pombe) (WEE1) in HGC27 cells was confirmed using co-immunoprecipitation and indirect immunofluorescence. The expression level of RACK1 in GC was significantly lower compared with that in pericarcinous tissues (P |
| Databáze: | OpenAIRE |
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