The Culture Dish Surface Influences the Phenotype and Cytokine Production of Human Monocyte-Derived Dendritic Cells
Autor: | Alexander Sauter, Dag Heiro Yi, Yayan Li, Sabine Roersma, Silke Appel |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Adult Male lcsh:Immunologic diseases. Allergy medicine.medical_treatment T cell non-adherent culture plate monocyte-derived dendritic cells Population Immunology Cell Culture Techniques law.invention 03 medical and health sciences 0302 clinical medicine law medicine Immunology and Allergy Humans Secretion tolerogen education Original Research Aged CD86 education.field_of_study Chemistry Petri dish Dendritic Cells Middle Aged Antigens Differentiation cytokines Cell biology immunogen adhesion 030104 developmental biology Cytokine medicine.anatomical_structure Cell culture homotypic clusters Cytokines Female monocytes lcsh:RC581-607 CD80 030215 immunology |
Zdroj: | Frontiers in Immunology, Vol 10 (2019) Frontiers in Immunology |
ISSN: | 1664-3224 |
DOI: | 10.3389/fimmu.2019.02352/full |
Popis: | Monocyte-derived dendritic cells (moDC) are an important scientific and clinical source of functional dendritic cells (DC). However, the optimization of the generation process has to date mainly been limited to the variation of soluble factors. In this study, we investigated the impact of the cell culture dish surface on phenotype and cytokine profile. We compared a standard cell culture dish to a non-adherent culture dish for two immunogenic maturation conditions, two tolerogenic conditions, and an unstimulated control. Phenotype, cytokine profile and T cell stimulatory capacity were determined after a 3-day culture. Light microscopy revealed an increase in homotypic cluster formation correlated with the use of non-adherent surfaces, which could be reduced by using blocking antibodies against CD18. All surface markers analyzed showed moderate to strong differences depending on the culture dish surface, including significantly decreased expression of key maturation markers such as CD80, CD86, and CCR7 as well as PD-L1 on cells stimulated with the Jonuleit cytokine cocktail cultured on a non-adherent surface. Significant differences in the secretion of many cytokines were observed, especially for cells stimulated with LPS, with over 10-fold decreased secretion of IL-10, IL12-p40, and TNF-α from the cells cultured on the non-adherent surface. All immunogenic moDC populations showed similar capacity to induce antigen-specific T cells. These results provide evidence that the DC phenotype depends on the surface used during moDC generation. This has important implications for the optimization of DC-based immunotherapy development and underlines that the local surrounding can interfere with the final DC population beyond the soluble factors. publishedVersion |
Databáze: | OpenAIRE |
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