The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast
| Autor: | Kiran J. van der Laan, Charles Mignon, Mayeul Chipaux, Thamir Hamoh, Axel Hochstetter, Felipe Perona Martinez, Aryan Morita, Alina Sigaeva, Romana Schirhagl |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
cell division Cell division General Chemical Engineering Saccharomyces cerevisiae ved/biology.organism_classification_rank.species 02 engineering and technology engineering.material yeast Article lcsh:Chemistry 03 medical and health sciences Asymmetric cell division General Materials Science Biotechnology research fluorescent nanodiamonds Model organism biology ved/biology Diamond 021001 nanoscience & nanotechnology biology.organism_classification Fluorescence Yeast 030104 developmental biology lcsh:QD1-999 Biophysics engineering 0210 nano-technology |
| Zdroj: | Nanomaterials Volume 10 Issue 3 Nanomaterials, Vol 10, Iss 3, p 516 (2020) Nanomaterials (Basel, Switzerland), 10(3):516. MDPI AG |
| ISSN: | 2079-4991 |
| DOI: | 10.3390/nano10030516 |
| Popis: | Fluorescent nanodiamonds are frequently used as biolabels. They have also recently been established for magnetic resonance and temperature sensing at the nanoscale level. To properly use them in cell biology, we first have to understand their intracellular fate. Here, we investigated, for the first time, what happens to diamond particles during and after cell division in yeast (Saccharomyces cerevisiae) cells. More concretely, our goal was to answer the question of whether nanodiamonds remain in the mother cells or end up in the daughter cells. Yeast cells are widely used as a model organism in aging and biotechnology research, and they are particularly interesting because their asymmetric cell division leads to morphologically different mother and daughter cells. Although yeast cells have a mechanism to prevent potentially harmful substances from entering the daughter cells, we found an increased number of diamond particles in daughter cells. Additionally, we found substantial excretion of particles, which has not been reported for mammalian cells. We also investigated what types of movement diamond particles undergo in the cells. Finally, we also compared bare nanodiamonds with lipid-coated diamonds, and there were no significant differences in respect to either movement or intracellular fate. |
| Databáze: | OpenAIRE |
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