Pseudomonas aeruginosa Type III Secretion System Virulotypes and Their Association with Clinical Features of Cystic Fibrosis Patients
| Autor: | Ana Judith Pires Garcia Quaresma, Karla Valéria Batista Lima, Ismari Perini Furlaneto, Luana Nepomuceno Godim Costa Lima, Giulia Leão da Cunha Brabo, Edilene do Socorro Nascimento Falcão Sarges, Marcos Vinicios Hino de Melo, Kátia Cilene Machado Lopes, Yan Corrêa Rodrigues |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Spirometry exoT medicine.medical_specialty exoS exoU 030106 microbiology exoY Virulence Infec??es por Pseudomonas / microbiologia Fibrose C?stica type III secretory system medicine.disease_cause Cystic fibrosis Gastroenterology Type three secretion system 03 medical and health sciences Virul?ncia 0302 clinical medicine Internal medicine medicine Pharmacology (medical) 030212 general & internal medicine Pathogen Original Research Pharmacology medicine.diagnostic_test business.industry Pseudomonas aeruginosa virulence genes medicine.disease Chronic infection Infectious Diseases Infection and Drug Resistance Sputum Pseudomonas aeruginosa / patogenicidade medicine.symptom business |
| Zdroj: | Infection and Drug Resistance Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1178-6973 |
| Popis: | This research was supported by funding from Funda??o de Amparo ? Pesquisa do Par?/Universidade do Estado do Par? (FAPESPA/UEPA) [Cooperation grant N?004/2019], Programa Institucional de Bolsas de Inicia??o Cient?fica/ Funda??o Amaz?nia Paraense de Amparo ? Pesquisa/ Universidade do Estado do Par? (FAPESPA/UEPA) [Cooperation grant N?001/2017/PPGBPA], Programa Institucional de Bolsas de Inicia??o Cient?fica/Funda??o Amaz?nia Paraense de Amparo ? Pesquisa/Universidade do Estado do Par? (FAPESPA/UEPA) [Cooperation grant N?002/2019/Gradua??o], Programa Institucional de Bolsas de Inicia??o Cient?fica/Instituto Evandro Chagas ? Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (PIBIC/IEC/CNPq) and Instituto Evandro Chagas/Minist?rio da Sa?de/Secretaria de Vigil?ncia em Sa?de (IEC/MS/SVS). Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil / Universidade Federal do Par?. Hospital Universit?rio Jo?o de Barros Barreto. Bel?m, PA, Brazil. Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil. Centro Universit?rio do Par?. Mestrado Profissional em Ensino em Sa?de ? Educa??o M?dica. Bel?m, PA, Brazil. Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade Federal do Par?. Hospital Universit?rio Jo?o de Barros Barreto. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Purpose: Pseudomonas aeruginosa appears as the main pathogen in cystic fibrosis (CF) involved in recurrent pneumonia and pulmonary exacerbations. The type III secretion system (T3SS) is one of its main determinants of virulence and is associated with poor clinical progression and increased mortality. This study determined the relationship of clinical features of patients with CF and P. aeruginosa T3SS virulotypes. Materials and Methods: From January 2018 to March 2019, P. aeruginosa were isolated from sputum and/or oropharyngeal swabs. T3SS markers (exoS, exoU, exoT and exoY) were detected by PCR. Clinical severity according to Shwachman-Kulckycki score and spirometry data were associated with T3SS virulotypes. Results: A total of 49 patients had positive cultures for P. aeruginosa. T3SS virulence-related markers were detected as follows: exoS 97.9% (n=48), exoU 63.2% (n=31), exoT 95.9% (n=47) and exoY 97.9% (n=48). The prevalence of exoS+/exoU+ virulotype was higher than previously reported in CF settings, being detected in 61.2% of the evaluated isolates, present in 70% of intermittent infections and with a significantly higher frequency in cases of exacerba-tions. The presence of exoU in chronic infection was not associated with poor clinical results. In chronic infections, the exoS+/exoU? virulotype prevailed (77.8%) and was associated to worse clinical results according to the Shwachman-Kulckycki score and spirometric. Conclusion: Our findings revealed a high prevalence of the atypical exoS+ /exoU+ virulo-type among P. aeruginosa isolates from patients with CF, which was associated with intermittent infection and early clinical alterations, while the exoS+ /exoU? virulotype was associated with chronic infection and worse clinical results. Finally, the presented data highlight the relevance of T3SS virulence markers in the clinical progression and disease severity in CF patients. |
| Databáze: | OpenAIRE |
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