THE DYNAMIC OF THE ENERGY METABOLISM OF THE CELLS OF WHITE RATS SKIN CONNECTIVE TISSUE UNDER CONDITIONS OF THE BURN INJURY AND HYPERGLYCEMIA

Autor: I.N. Ryzhko, Yu.S. Osadchuk, L.V. Natrus, N.P. Verko, T.I. Panova
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Медична наука України, Vol 14, Iss 1-2, Pp 3-10 (2018)
ISSN: 2664-4738
Popis: Relevance. Macrophages exist in the wound in several phenotypic states, and the effect of these cells on intercellular co-operation at each stage of tissue repair depends on their specific phenotype. Depending on the microenvironment, they can quickly change their functional phenotype from proinflammatory M1 to anti-inflammatory M2, and vice versa. However, the effect of the functional phenotype of macrophages on cellular metabolism is not unilateral, the change in the features of metabolism, especially energy, also significantly affects the phenotype of cells. Objective. The aim of this work is to analyze the oxidoreductases activity of neutrophils, macrophages and fibroblasts in the connective tissue of the white rats skin under conditions of local thermal damage when there is a carbohydrate metabolism violation to predict the efficiency of the tissue regeneration during the repair process. Material and methods. There were carried out the histochemical and pathomorphological researches of the skin burn wound healing dynamic (3, 7, 14, 21 days) of the white mature male rats (Wistar line) with the weight of 180-210g without somatic pathology (control group, n=28) and with stable uncorrected hyperglycemia modeled by streptozotocin (n=28). It has been carried out the histochemical study of oxidoreductase enzymes activity on the cryostat sections (10 μm thickness): succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), NADH- dehydrogenase (NADH-DH). The histochemical index of the oxidoreductase enzymes activity was being calculated by the semi-quantitative method. Results. The study of the cells’ quantitative changes in the connective tissue regenerate and the reactions of energy metabolism against the background of hyperglycemia has some features. The consistency of the connective tissue infiltration by the leukocytes of different types during reparative regeneration after burn injury was being happened in a classical scenario with a quantitative predominance of cells in a group of animals without somatic pathology that points to the reduction of blood reactivity under conditions of glycemia. In the dynamics of the healing process the change of the LDH and NADH-DH activity in the macrophages and fibroblasts had a tendency to increase on the 7th and 14th days of the experiment and differed significantly during observation from the parameters in the 3d day (p
Databáze: OpenAIRE