Transcriptional analysis of muscle tissue and isolated satellite cells in spastic cerebral palsy
| Autor: | Stephanie K Lee, Robert E. Akins, Erin L. Crowgey, Karyn G. Robinson |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Muscle tissue 030506 rehabilitation Adolescent Satellite Cells Skeletal Muscle Biology Cerebral palsy Transcriptome Andrology 03 medical and health sciences 0302 clinical medicine Spastic cerebral palsy Developmental Neuroscience Gene expression medicine Humans Myocyte RNA-Seq Child Muscle Skeletal Myogenesis Cerebral Palsy Gene Expression Profiling Skeletal muscle medicine.disease medicine.anatomical_structure Muscle Spasticity Case-Control Studies Pediatrics Perinatology and Child Health Female Neurology (clinical) 0305 other medical science 030217 neurology & neurosurgery |
| Zdroj: | Developmental Medicine & Child Neurology. 63:1213-1220 |
| ISSN: | 1469-8749 0012-1622 |
| DOI: | 10.1111/dmcn.14915 |
| Popis: | AIM To analyze transcriptomes from muscle tissue and cells to improve our understanding of differences in gene expression and molecular function in cerebral palsy (CP) muscle. METHOD In this case-control study, eight participants with CP (five males, three females; mean [SD] age 14y 2mo [1y 8mo]) and 11 comparison individuals (eight males, three females; mean [SD] age 14y 0mo [2y 6mo]) were enrolled after informed consent/assent and skeletal muscle was obtained during surgery. RNA was extracted from tissue and from primary satellite cells grown to form myotubes in vitro. RNA sequencing data were analyzed using validated informatics pipelines. RESULTS Analysis identified expression of 6308 genes in the tissue samples and 7459 in the cultured cells. Significant differential expression between CP and control was identified in 87 genes in the tissue and 90 genes in isolated satellite cell-derived myotube cultures. INTERPRETATION Both tissue and cell analyses identified differential expression of genes associated with muscle development and multiple pathways of interest. What this paper adds Expression differences were found in muscle tissue and in isolated muscle cells. There was low variability in expression among cells isolated from different muscles. Expression differences suggest complex functional alterations in spastic cerebral palsy. |
| Databáze: | OpenAIRE |
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