Localization of vascular endothelial growth factor in synovial membrane mast cells: examination with 'multi-labelling subtraction immunostaining'
| Autor: | Kohji Miyazaki, Hiroyuki Yakushiji, Ladias Robert, Motohiro Sawatsubashi, Michito Mori, Takahiro Yamada, Genichiro Edakuni, Yumi Itoh |
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| Rok vydání: | 1998 |
| Předmět: |
Vascular Endothelial Growth Factor A
Pathology medicine.medical_specialty Cell type medicine.medical_treatment Pannus Endothelial Growth Factors Pathology and Forensic Medicine Arthritis Rheumatoid chemistry.chemical_compound Chymases medicine Humans Mast Cells Molecular Biology Inflammation Lymphokines Vascular Endothelial Growth Factors business.industry Growth factor Serine Endopeptidases Synovial Membrane Cell Biology General Medicine Mast cell medicine.disease Immunohistochemistry Vascular endothelial growth factor medicine.anatomical_structure Cytokine chemistry Tryptases Synovial membrane business Immunostaining |
| Zdroj: | Virchows Archiv. 433:567-570 |
| ISSN: | 1432-2307 0945-6317 |
| DOI: | 10.1007/s004280050290 |
| Popis: | Mast cells are believed to play a novel part in the development of destructive synovial pannus in rheumatoid arthritis (RA). This study was undertaken to investigate the localization of vascular endothelial growth factor (VEGF) in the synovial membrane using a unique immunostaining technique. Synovial specimens of RA patients were examined immunohistochemically and were compared with specimens from non-RA controls. Multi-labelling subtraction immunostaining, a modification of double- and triple-labelling immunostaining, revealed that the VEGF-positive cells were identical to tryptase-positive cells (mast cells). No other cell types were found to be positive for VEGF. The synovium of RA patients showed a larger number of VEGF-positive mast cells than that of non-RA controls (P |
| Databáze: | OpenAIRE |
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