Diffuse High Intensity PD–L1 Staining in Thymic Epithelial Tumors
| Autor: | Sukhmani K. Padda, Erich J. Schwartz, Heather A. Wakelee, Lu Tian, Joel W. Neal, Jonathan W. Riess, Robert B. West, Holbrook E Kohrt |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Adolescent Thymus Gland B7-H1 Antigen Article Immunoenzyme Techniques Young Adult PD-L1 Biomarkers Tumor Medicine Humans Neoplasms Glandular and Epithelial Child Survival rate Aged Neoplasm Staging Aged 80 and over Tissue microarray biology business.industry Hazard ratio Histology Thymus Neoplasms Middle Aged Prognosis 3. Good health Staining Survival Rate Oncology Tissue Array Analysis Case-Control Studies Child Preschool biology.protein Immunohistochemistry Female Neoplasm Grading business Follow-Up Studies |
| Zdroj: | Journal of Thoracic Oncology. 10(3):500-508 |
| ISSN: | 1556-0864 |
| DOI: | 10.1097/jto.0000000000000429 |
| Popis: | Introduction Blockade of the immune checkpoint programmed death receptor ligand-1 (PD-L1)/PD-1 pathway has well-established clinical activity across many tumor types. PD-L1 protein expression by immunohistochemistry is emerging as a predictive biomarker of response to these therapies. Here, we examine PD-L1 expression in a thymic epithelial tumor (TET) tissue microarray (TMA). Methods The TMA contained 69 TETs and 17 thymic controls, with each case represented by triplicate cores. The TMA was stained with rabbit monoclonal antibody (clone 15; Sino Biological, Beijing, China) to human PD-L1. PD-L1 staining was scored based on intensity as follows: 0=none, 1=equivocal/uninterpretable, 2=weak, and 3=intermediate-strong. Those cases with all cores scoring three in the epithelial component were categorized as PD-L1high and the remaining as PD-L1low. Results PD-L1high scores were more frequent in TETs than in controls (68.1% versus 17.6%; p = 0.0036). PD-L1 scores and histology were significantly correlated, with higher intensity staining in World Health Organization (WHO). B2/B3/C TETs. Only 14.8% of TETs had PD-L1 staining of associated lymphocytes. In an adjusted analysis (age/sex), PD-L1high TETs had a significantly worse overall survival (hazard ratio: 5.40, 95% confidence interval: 1.13–25.89; p = 0.035) and a trend for worse event-free survival (hazard ratio: 2.94, 95% confidence interval: 0.94–9.24; p = 0.064). Conclusions PD-L1 expression was present in all cases of TETs within the epithelial component but only in a minority in the lymphocytic component. TETs stained more intensely for PD-L1 than in controls, and PD-L1high TETs were associated with more aggressive histology and worse prognosis. This study lends rationale to a clinical trial with anti-PD-1/PD-L1 therapy in this rare tumor type. |
| Databáze: | OpenAIRE |
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