Rapid degradation of ferulic acid via 4-vinylguaiacol and vanillin by a newly isolated strain of Bacillus coagulans
| Autor: | Hemraj Nandanwar, K. G. Gupta, R M Vohra, Prateek Sharma, B. Karmakar, Ranbir Chander Sobti |
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| Rok vydání: | 2000 |
| Předmět: |
Magnetic Resonance Spectroscopy
Coumaric Acids Bacillus Bioengineering Applied Microbiology and Biotechnology High-performance liquid chromatography Protocatechuic acid Trees Ferulic acid chemistry.chemical_compound Vanillic acid Organic chemistry Chromatography High Pressure Liquid Chromatography biology Vanillin Guaiacol General Medicine Biodegradation biology.organism_classification Wood Metabolic pathway Biodegradation Environmental chemistry Benzaldehydes Bacillus coagulans Biotechnology |
| Zdroj: | Journal of Biotechnology. 80:195-202 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/s0168-1656(00)00248-0 |
| Popis: | A new strain Bacillus coagulans BK07 was isolated from decomposed wood-bark, based on its ability to grow on ferulic acid as a sole carbon source. This strain rapidly decarboxylated ferulic acid to 4-vinylguaiacol, which was immediately converted to vanillin and then oxidized to vanillic acid. Vanillic acid was further demethylated to protocatechuic acid. Above 95% substrate degradation was obtained within 7 h of growth on ferulic acid medium, which is the shortest period of time reported to date. The major degradation products, was isolated and identified by thin-layer chromatography, high performance liquid chromatography and 1H-nuclear magnetic resonance spectroscopy were 4-vinylguaiacol, vanillin, vanillic acid and protocatechuic acid. |
| Databáze: | OpenAIRE |
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