Automatic motion compensation for structured illumination endomicroscopy using a flexible fiber bundle
| Autor: | Michael Hughes, Andrew D. Thrapp |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Paper
Laser scanning Optical sectioning Computer science Biomedical Engineering mosaicking Image registration Field of view 01 natural sciences Digital micromirror device law.invention 010309 optics Biomaterials Motion Computer Systems law 0103 physical sciences Image Processing Computer-Assisted Endomicroscopy Fiber Optic Technology Computer vision QC355 digital micromirror device Pattern orientation Lighting Microscopy Motion compensation business.industry Equipment Design endomicroscopy Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials motion compensation structured illumination Artificial intelligence Artifacts business |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.jbo.25.2.026501 |
| Popis: | Significance: Confocal laser scanning enables optical sectioning in clinical fiber bundle endomicroscopes, but lower-cost, simplified endomicroscopes use widefield incoherent illumination instead. Optical sectioning can be introduced in these simple systems using structured illumination microscopy (SIM), a multiframe digital subtraction process. However, SIM results in artifacts when the probe is in motion, making the technique difficult to use in vivo and preventing the use of mosaicking to synthesize a larger effective field of view (FOV).\ud \ud Aim: We report and validate an automatic motion compensation technique to overcome motion artifacts and allow generation of mosaics in SIM endomicroscopy.\ud \ud Approach: Motion compensation is achieved using image registration and real-time pattern orientation correction via a digital micromirror device. We quantify the similarity of moving probe reconstructions to those acquired with a stationary probe using the relative mean of the absolute differences (MAD). We further demonstrate mosaicking with a moving probe in mechanical and freehand operation.\ud \ud Results: Reconstructed SIM images show an improvement in the MAD from 0.85 to 0.13 for lens paper and from 0.27 to 0.12 for bovine tissue. Mosaics also show vastly reduced artifacts.\ud \ud Conclusion: The reduction in motion artifacts in individual SIM reconstructions leads to mosaics that more faithfully represent the morphology of tissue, giving clinicians a larger effective FOV than the probe itself can provide. |
| Databáze: | OpenAIRE |
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