Suppression Of Hepatitis C Virus Core Protein By Short Hairpin Rna Expression Vectors In The Core Protein Expression Huh-7 Cells

Autor:	Hiroyasu Kaneko, Naoko Miyano-Kurosaki, Hiroshi Takaku, Hitoshi Suzuki, Nobushige Tamai, Kunitada Shimotohno
Rok vydání:	2007
Předmět:	Hepatitis C virus Genetic Vectors Hepacivirus GFP Virus Replication medicine.disease_cause hepatitis C virus (HCV) core protein Biochemistry Article Small hairpin RNA RNA interference Cell Line Tumor Gene expression Genetics medicine Protein biosynthesis DsRed Humans Short hairpin RNAs (shRNAs) RNA Messenger RNA Small Interfering Regulation of gene expression Huh-7 cells Expression vector Chemistry Viral Core Proteins virus diseases General Medicine Molecular biology digestive system diseases Gene Expression Regulation Viral replication RNAi Molecular Medicine
Zdroj:	Nucleosides, Nucleotides & Nucleic Acids
ISSN:	1532-2335 1525-7770
Popis:	Short hairpin RNAs (shRNAs) efficiently inhibit gene expression by RNA interference. Here, we report the efficient inhibition by DNA-based vector-derived shRNAs of core protein expression in Huh-7 cells. The shRNAs were designed to target the core region of the hepatitis C virus (HCV) genome. The core region is the most conserved region in the HCV genome, making it an ideal target for shRNAs. We identified an effective site on the core region for suppression of the HCV core protein. The HCV core protein in core protein-expressing Huh-7 cells was downregulated by core protein-shRNA expression vectors (core-shRNA-452, 479, and 503). Our results support the feasibility of using shRNA-based gene therapy to inhibit HCV core protein production.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a476d4720bcba3e1e9b8716e187fefb https://doi.org/10.1080/15257770701503787 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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