Sirtuin 6 mediated stem cell cardiomyogenesis on protein coated nanofibrous scaffolds
| Autor: | Venkatraman Ravi, Aditi Jain, Nagalingam R. Sundaresan, Arpana Gopi Panicker, Kaushik Chatterjee, Lopamudra Das Ghosh |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
SIRT6
Organogenesis Polyesters Nanofibers Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering 02 engineering and technology Calcium in biology Extracellular matrix 03 medical and health sciences Centre for Biosystems Science and Engineering Humans Sirtuins Myocytes Cardiac General Materials Science Wnt Signaling Pathway Cells Cultured Cell Proliferation 030304 developmental biology Microbiology & Cell Biology 0303 health sciences Tissue Scaffolds biology Chemistry Stem Cells Mesenchymal stem cell Wnt signaling pathway Materials Engineering (formerly Metallurgy) Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Cell biology Nanofiber Sirtuin biology.protein Molecular Medicine Calcium Stem cell 0210 nano-technology Biomarkers |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 19:145-155 |
| ISSN: | 1549-9634 |
| DOI: | 10.1016/j.nano.2019.03.005 |
| Popis: | The cellular niche provides combination of biomolecular and biophysical cues to control stem cell fate. Three-dimensional (3D) aligned nanofibrous scaffolds can effectively augment stem cell cardiomyogenesis. This work aims to understand the role of biomolecular signals from extracellular matrix (ECM) proteins and leverage them to further promote cardiomyogenesis on nanofibrous scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on 3D aligned polycaprolactone scaffolds coated with different ECM proteins. Among multiple coatings tested, collagen coated fibers were most effective in promoting cardiomyogenesis as determined from increased expression of cardiac biomarkers and intracellular calcium flux. At molecular level, enhanced differentiation on collagen coated fibers was associated with an increased level of sirtuin 6 (SIRT6). Depletion of SIRT6 using siRNA attenuated the differentiation process through activation of Wnt signaling pathway. This study, thus, demonstrates that protein coated scaffolds can augment cardiomyogenic differentiation of stem cells through a combination of topographical and biomolecular signals. |
| Databáze: | OpenAIRE |
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