Three Dimensional Structural Modelling of Lipase Encoding Gene from Soil Bacterium Alcaligenes sp. JG3 Using Automated Protein Homology Analysis

Autor:	Endang Astuti, Dilin Rahayu Nataningtyas, Tri Joko Raharjo
Rok vydání:	2019
Předmět:	lipase gene Alcaligenes faecalis biology Sequence analysis Chemistry In silico ATP-binding cassette transporter General Chemistry biology.organism_classification LipJG3 ABC transporter protein Biochemistry Catalytic triad biology.protein Alcaligenes Lipase QD1-999 Gene
Zdroj:	Indonesian Journal of Chemistry, Vol 19, Iss 3, Pp 565-574 (2019) Indonesian Journal of Chemistry; Vol 19, No 3 (2019); 565-574
ISSN:	2460-1578 1411-9420
DOI:	10.22146/ijc.34152
Popis:	Bacterial lipases have significant potential to be used as the biocatalyst for many chemical reactions. In this study, a novel gene encoding lipase was isolated from an Alcaligenes sp. JG3. A pair of designed primer has successfully isolated 1 kb (LipJG3) that shares 98% identity towards lipase from Alcaligenes faecalis during sequence analysis. By using in silico tools, LipJG3 was related to the transporter protein sequences. Three highly conserved regions consisting of EASGSKT, VILLD, and LSGGQQQRVAIA were found. These regions were known as ATP-binding signature at Walker-A and Walker-B motifs and the S signature of ABC transporter family respectively. In addition, the 3-D structure of LipJG3 has been suggested but the role of the catalytic triad residues have been not fully understood.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a42c73a5e62ce4b549b2bb0266c548b https://doi.org/10.22146/ijc.34152 Zobrazit plný text záznamu