Type II collagen‐positive embryonic progenitors are the major contributors to spine and intervertebral disc development and repair
| Autor: | Shuying Yang, Shuting Yang, Xinhua Li, Ling Qin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Medicine (General) Stromal cell type II collagen Type II collagen Intervertebral Disc Degeneration Biology lineage‐tracing Extracellular matrix 03 medical and health sciences Mice 0302 clinical medicine R5-920 Tissue‐specific Progenitor and Stem Cells medicine Animals Collagen Type II QH573-671 Cartilage nucleus pulposus Mesenchymal stem cell Intervertebral disc Cell Differentiation Cell Biology General Medicine musculoskeletal system Embryonic stem cell Cell biology stem cell 030104 developmental biology medicine.anatomical_structure intervertebral disc Stem cell Cytology 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine Stem Cells Translational Medicine, Vol 10, Iss 10, Pp 1419-1432 (2021) |
| ISSN: | 2157-6580 2157-6564 |
| Popis: | Basic mechanism of spine development is poorly understood. Type II collagen positive (Col2+) cells have been reported to encompass early mesenchymal progenitors that continue to become chondrocytes, osteoblasts, stromal cells, and adipocytes in long bone. However, the function of Col2+ cells in spine and intervertebral disc (IVD) development is largely unknown. To further elucidate the function of Col2+ progenitors in spine, we generated the mice with ablation of Col2+ cells either at embryonic or at postnatal stage. Embryonic ablation of Col2+ progenitors caused the mouse die at newborn with the absence of all spine and IVD. Moreover, postnatal deletion Col2+ cells in spine resulted in a shorter growth plate and endplate cartilage, defected inner annulus fibrosus, a less compact and markedly decreased gel‐like matrix in the nucleus pulposus and disorganized cell alignment in each compartment of IVD. Genetic lineage tracing IVD cell populations by using inducible Col2‐creERT;tdTomato reporter mice and non‐inducible Col2‐cre;tdTomato reporter mice revealed that the numbers and differentiation ability of Col2+ progenitors decreased with age. Moreover, immunofluorescence staining showed type II collagen expression changed from extracellular matrix to cytoplasm in nucleus pulposus between 6 month and 1‐year‐old mice. Finally, fate‐mapping studies revealed that Col2+ progenitors are essential for IVD repair in IVD injured model. In summary, embryonic Col2+ cells are the major source of spine development and Col2+ progenitors are the important contributors for IVD repair and regeneration. Type II collagen‐positive embryonic progenitors are the major contributors to spine and intervertebral disc development and repair. |
| Databáze: | OpenAIRE |
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