IMMUNOHISTOLOGICAL INVESTIGATION OF EPITHELIAL COMPONENTS OF ADENOLYMPHOMA OF PAROTID GLAND
| Autor: | Itirou Makino, Masataka Murakami, Saburo Mimaki, Yasushi Murakami, Yasuyuki Nishiyama |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Pathology medicine.medical_specialty Vimentin Columnar Cell Biology Epithelium Basal (phylogenetics) stomatognathic system Acinus medicine Humans Parotid Gland Adenolymphoma S100 Proteins Myoepithelial cell Immunohistochemistry Actins Parotid Neoplasms Parotid gland medicine.anatomical_structure Otorhinolaryngology biology.protein Keratins Female Duct (anatomy) |
| Zdroj: | Nippon Jibiinkoka Gakkai Kaiho. 95:526-534 |
| ISSN: | 1883-0854 0030-6622 |
| DOI: | 10.3950/jibiinkoka.95.526 |
| Popis: | In order to develop a more objective method of evaluating the origin of adenolymphoma, we immunohistochemically investigated the expression of cytokeratins, vimentin, S-100 protein, and α-smooth-muscle actin using the avidin-biotin-peroxidase (ABC) method in ethanol-fixed paraffin-embedded specimens from 8 adenolymphomas. Several kinds of monoclonal antibodies which react monospecifically with each subclass of cytokeratins were used. Results were compared with specimens of 8 normal parotid glands by radical neck dissection in patients with other diseases who had not undergone radiotherapy. In the adenolymphoma specimens, basal cells were strongly positive for CK-6, but reactivity of columnar cells was apparently reduced. In contrast, columnar cells were strongly positive for CK-7, but reactivity of basal cells was reduced. In normal parotid gland specimens, CK-7 was also detected in all columnar cells in the ductal system, although some duct cells around the columnar cells, which showed strong CK-6 expression, showed poor CK-7 expression. α-smooth-muscle actin was present in myoepithelial cells in normal parotid gland specimens, but not in basal cells of adenolymphoma or of the normal ductal system, which were CK-6-positive. As these characteristic findings were consistently observed in all specimens, the demonstration of CK-6, CK-7, and α-smooth-muscle actin may be useful in the recognition and classification of columnar and basal cells. CK-8 was present in both columnar and basal cells of adenolymphoma and of the normal duct, but in the normal parotid gland, acinus cells were also strongly positive for CK-8. On the other hand, acinus cells in normal parotid glands were negative for CK-19, although both columnar and basal cells of adenolymphoma and of the normal duct were strongly positive for CK-19. The expression patterns of CK-3, -13, and -18 were similar to that of CK-19. All cells in adenolymphoma and normal parotid gland were uniformly negative for CK-1, -4, -10, -11, and -14, vimentin, and S-100 protein.In adenolymphoma, staining patterns of all cytokeratins and other proteins investigated generally resembled their distribution in the corresponding portion of the normal parotid gland ductal system. Since our results revealed no differences which would support a bi-cellular theory regarding the origin of adenolymphoma, we suggest that basal cells, which can be recognized by CK-6 expression in the ductal systems of normal parotid glands, should be investigated further to determine the origin of adenolymphoma. |
| Databáze: | OpenAIRE |
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