Basophil Activation Test for Allergy Diagnosis
| Autor: | María José Torres, Tahia D. Fernandez, Inmaculada Doña, Adriana Ariza |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Drug
Allergy media_common.quotation_subject General Chemical Engineering Drug allergy Basophil Immunoglobulin E medicine.disease_cause General Biochemistry Genetics and Molecular Biology Allergen Hypersensitivity medicine Humans Skin Tests media_common biology CD63 General Immunology and Microbiology Diagnostic Tests Routine Tetraspanin 30 business.industry General Neuroscience Allergens medicine.disease Basophils Basophil activation medicine.anatomical_structure Immunology biology.protein business |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/62600-v |
| Popis: | The basophil activation test (BAT) is a complementary in vitro diagnostic test that can be used in addition to clinical history, skin test (ST), and specific IgE (sIgE) determination in the evaluation of IgE-mediated allergic reactions to food, insect venom, drugs, as well as some forms of chronic urticaria. However, the role of this technique in the diagnostic algorithms is highly variable and not well determined. BAT is based on the determination of basophil response to allergen/drug cross-linking IgE activation through the measurement of activation markers (such as CD63, CD203c) by flow cytometry. This test can be a useful and complementary tool to avoid controlled challenge tests to confirm allergy diagnosis, especially in subjects experiencing severe life-threatening reactions. In general, the performance of BAT should be considered if i) the allergen/drug produces false positive results in ST; ii) there is no allergen/drug source to use for ST or sIgE determination; iii) there is discordance between patient history and ST or sIgE determination; iv) symptoms suggest that ST may result in systemic response; v) before considering a CCT to confirm the culprit allergen/drug. The main limitations of the test are related to non-optimal sensitivity, especially in drug allergy, the need to perform the test no longer than 24 h after sample extraction, and the lack of standardization between laboratories in terms of procedures, concentrations, and cell markers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|