Comparison of media for enumeration of Clostridium perfringens from foods

Autor: Frans M. Rombouts, J. Marugg, A. Zicavo, R.R. Beumer, P.H. in 't Veld, G. Wörner, T. Kärkkäinen, T. Johansson, G.P. Eijhusen, A.E.I. de Jong, F.H.M. Warmerdam, M. Grand, E.J.F. Brouwer-Post
Rok vydání: 2003
Předmět:
sporulation
Enumeration
Clostridium perfringens
bacterial count
Colony Count
Microbial
Pilot Projects
medicine.disease_cause
Levensmiddelenmicrobiologie
agar medium
Clostridium
iron
sulfite
Agar
Food science
comparative study
BU Microbiological & Chemical Food Analysis
Spores
Bacterial
Cycloserine
article
cycloserine
Milk
priority journal
sugar
food contamination
bacterium contamination
medicine.drug
Biotechnology
Microbiology (medical)
Media
food.ingredient
culture medium
oleandomycin
Biology
Microbiology
Agar plate
food
Sulfadiazine
azide
bacterium isolation
medicine
Food microbiology
Animals
controlled study
Molecular Biology
VLAG
agar
Oleandomycin
nonhuman
Biology Nutrition
food analysis
sulfadiazine
polymyxin
biology.organism_classification
bacterial strain
Culture Media
bacterium colony
Food Microbiology
BU Microbiologische & Chemische Voedselanalyse
microbiological examination
Zdroj: Journal of Microbiological Methods, 3, 54, 359-366
Journal of Microbiological Methods, 54(3), 359-366
Journal of Microbiological Methods 54 (2003) 3
ISSN: 0167-7012
Popis: Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens (SFP) agar, sulfite cycloserine azide (SCA), differential clostridial agar (DCA), and oleandomycin polymyxin sulfadiazine perfringens (OPSP) agar] were compared in a prestudy, of which four (IS, TSC, SCA, and DCA) were selected for an international collaborative trial. Recovery of 15 pure strains was tested in the prestudy and recovery of one strain from foodstuffs was tested in the collaborative trial. Results from the prestudy did reveal statistical difference of the media but recoveries on all media were within the microbiological limits (+/- 30%) of IS, which was set as a reference medium. Recoveries on the media tested in the collaborative trial were statistically different as well, but these differences were of no microbiological-analytical relevance. Food matrices did not affect the recovery of C perfringens in general. DCA and SCA, in particular, are labor-intensive to prepare and DCA frequently failed to produce black colonies; gray colonies were quite common. Since IS medium is nonselective, it was concluded that TSC was the most favorable medium for the enumeration of C. perfringens from foods. (C) 2003 Elsevier Science B.V. All rights reserved.
Databáze: OpenAIRE
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