The use of a multiplex real-time PCR assay for diagnosing acute respiratory viral infections in children attending an emergency unit

Autor: E. Grouteau, Isabelle Claudet, Jacques Izopet, A. Pierre, Catherine Mengelle, Jean-Michel Mansuy, Karine Sauné, Pascale Micheau
Přispěvatelé: CHU Purpan, Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], CHU Toulouse [Toulouse], Embodiment, social ineQualities, lifecoUrse epidemiology, cancer and chronIc diseases, intervenTions, methodologY (Equipe 5 - EQUITY), Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Virologie [Toulouse]
Rok vydání: 2014
Předmět:
Male
viruses
Multiplex-PCR
ARI
acute respiratory infections
IV
influenza viruses
medicine.disease_cause
0302 clinical medicine
Multiplex
Prospective Studies
030212 general & internal medicine
Respiratory system
Child
Children
Respiratory Tract Infections
ComputingMilieux_MISCELLANEOUS
0303 health sciences
NAT
nucleic acid tests
Respiratory tract infections
biology
3. Good health
Infectious Diseases
Molecular Diagnostic Techniques
Virus Diseases
Child
Preschool
Viruses
Respiratory
Emergency Medicine
Rhinovirus
Emergency Service
Hospital
Adolescent
Spread
Real-Time Polymerase Chain Reaction
Article
Virus
03 medical and health sciences
RSV
respiratory syncytial viruses
MPV
human metapneumovirus
Human metapneumovirus
Virology
Multiplex polymerase chain reaction
medicine
Humans
Multiplex ligation-dependent probe amplification
[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics
030306 microbiology
Infant
Newborn
PiV
parainfluenza viruses
Infant
biology.organism_classification
RV
rhinovirus
Symptoms
Immunology
ADV
adenovirus
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
MPLA
multiplex ligation-dependent probe amplification
Multiplex Polymerase Chain Reaction
CoV
coronaviruses
Zdroj: Journal of Clinical Virology
Journal of Clinical Virology, Elsevier, 2014, 61 (3), pp.411-417
ISSN: 1386-6532
Popis: Highlights • Evaluate the use of multiplex real-time PCR for diagnosing respiratory infections. • 857/966 samples from 914 children were positive for one or multiple viruses. • Respiratory syncytial virus and rhinovirus were the most prevalent. • Co-infections were associated with severe respiratory symptoms. • The spread of respiratory viruses returned to the one it was before the flu outbreak.
Background The use of a multiplex molecular technique to identify the etiological pathogen of respiratory viral infections might be a support as clinical signs are not characteristic. Objectives The aim of the study was to evaluate a multiplex molecular real-time assay for the routine diagnosis of respiratory viruses, to analyze the symptoms associated with the pathogens detected and to determine the spread of virus during the period. Study design Respiratory samples were collected from children presenting with respiratory symptoms and attending the emergency unit during the 2010–2011 winter seasons. Samples were tested with the multiplex RespiFinder® 15 assay (PathoFinder™) which potentially detects 15 viruses. Results 857 (88.7%) of the 966 samples collected from 914 children were positive for one (683 samples) or multiple viruses (174 samples). The most prevalent were the respiratory syncytial virus (39.5%) and the rhinovirus (24.4%). Influenza viruses were detected in 139 (14.4%) samples. Adenovirus was detected in 93 (9.6%) samples, coronaviruses in 88 (9.1%), metapneumovirus in 51 (5.3%) and parainfluenzae in 47 (4.9%). Rhinovirus (40%) was the most prevalent pathogen in upper respiratory tract infections while respiratory syncytial virus (49.9%) was the most prevalent in lower respiratory tract infections. Co-infections were associated with severe respiratory symptoms. Conclusion The multiplex assay detected clinically important viruses in a single genomic test and thus will be useful for detecting several viruses causing respiratory tract disorders.
Databáze: OpenAIRE
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