E-NTPDase and E-ADA activities are altered in lymphocytes of patients with indeterminate form of Chagas' disease
| Autor: | Viviane do Carmo Gonçalves Souza, Karine Bizzi Schlemmer, Maria Rosa Chitolina Schetinger, Emerson André Casali, Carine Eloise Prestes Zimmermann, Cristiano Bicca Noal, Vera Maria Morsch, Daniela B.R. Leal, Jeandre Augusto dos Santos Jaques, Claudio A.M. Leal, Juliana Fleck |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Chagas disease Adenosine Deaminase Gene Expression Regulation Enzymologic Immune system Western blot Antigens CD Adenine nucleotide medicine Animals Humans Chagas Disease Nucleotide Lymphocytes Trypanosoma cruzi chemistry.chemical_classification biology medicine.diagnostic_test Apyrase Purinergic receptor Middle Aged medicine.disease biology.organism_classification Adenosine Infectious Diseases chemistry Case-Control Studies Immunology Female Parasitology medicine.drug |
| Zdroj: | Parasitology International. 61:690-696 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2012.07.008 |
| Popis: | Trypanosoma cruzi infection triggers a chronic inflammatory process in human host and purinergic system ecto-enzymes play an important role in modulating the inflammatory and immune responses. In this study, it was investigated ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and ecto-adenosine deaminase (E-ADA; EC 3.5.4.4) activities in lymphocytes from patients with indeterminate form of Chagas' disease (IFCD). Twenty-five IFCD patients and 25 healthy subjects (control group) were selected. The peripheral lymphocytes were isolated and E-NTPDase and E-ADA activities were determined. Adenine nucleotides and adenosine levels were determined in serum by HPLC and the E-NTPDase1 expression in lymphocytes by Western blot analysis. E-NTPDase (ATP and ADP as substrates) and E-ADA (adenosine as substrate) activities were decreased in lymphocytes from IFCD patients (P0.05 and P0.01, respectively), while the E-NTPDase1 expression presented no changes in these patients. Serum ATP levels showed to be decreased (P0.05) and both AMP (P0.01) and adenosine (P0.001) levels were increased in the IFCD group. The enzymatic alterations observed are in agreement with the immune response against T. cruzi infection in IFCD patients, since the decreased extracellular ATP and the increased adenosine levels trigger a Th2 anti-inflammatory response, which it is associated to adaptation of host to parasite, preventing clinical progress of disease. |
| Databáze: | OpenAIRE |
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