Diagnostic significance of immunoglobulin G avidity in symptomatic and asymptomatic West Nile virus infection

Autor: Vladimir Savić, Ljubo Barbić, Branimir Kristofic, Tatjana Vilibić-Čavlek, Bozana Miklausic, Giovanni Savini, Branko Kolarić, Tanja Potocnik-Hunjadi, Ljiljana Perić, Eddy Listeš, Sanja Zember, Irena Tabain, Vladimir Stevanović, Dario Sabadi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Microbiology (medical)
West Nile virus
diagnosis
IgG avidity
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962
viruses
030231 tropical medicine
Antibody Affinity
Enzyme-Linked Immunosorbent Assay
Antibodies
Viral
Asymptomatic
Immunoglobulin G
Serology
03 medical and health sciences
0302 clinical medicine
Blood serum
BIOMEDICINA I ZDRAVSTVO. Javno zdravstvo i zdravstvena zaštita
Diagnosis
medicine
Seroprevalence
Humans
Avidity
030212 general & internal medicine
biology
business.industry
BIOMEDICINE AND HEALTHCARE. Public Health and Health Care
virus diseases
BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Infectology
3. Good health
Infectious Diseases
Immunoglobulin M
Immunology
biology.protein
Parasitology
Seasons
Antibody
medicine.symptom
business
BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Infektologija
West Nile Fever
Zdroj: Revista da Sociedade Brasileira de Medicina Tropical v.51 n.5 2018
Revista da Sociedade Brasileira de Medicina Tropical
Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
Volume 51
Issue 5
Revista da Sociedade Brasileira de Medicina Tropical, Volume: 51, Issue: 5, Pages: 591-595, Published: OCT 2018
Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 591-595
ISSN: 0037-8682
1678-9849
Popis: INTRODUCTION: West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up to 500 days in certain patients. To evaluate the usefulness of immunoglobulin G (IgG) avidity assessment in the diagnosis of WNV infection, we analyzed 54 WNV IgM- and/or IgG-positive serum samples from 39 patients with neuroinvasive disease and 15 asymptomatic cases tested during a seroprevalence investigation. ----- METHODS: Serological tests (WNV IgM/IgG antibody detection, IgG avidity) were performed using commercially available enzyme-linked immunosorbent assays. ----- RESULTS: WNV IgM antibodies were detected in 47 (87%) samples. Acute/recent WNV infection was confirmed based on low/borderline avidity index (AI) in 44 IgM-positive samples (93.6%). In three IgM-positive samples (6.4%), high IgG AIs were detected, thus indicating persisting IgM antibodies from previous infections. All IgM-negative samples showed high AIs. Patients with WNV neuroinvasive disease tested within 30 days showed low AIs. In six patients tested 34-50 days after disease onset, AI was borderline (42%-60%), suggesting earlier WNV IgG maturation. Samples with the highest IgM values were associated with the lowest AIs (Spearman's rho coefficient -0.767, p < 0.001). ----- CONCLUSIONS: Our results indicate that IgG avidity differentiates current/recent WNV infection from persistent IgM seropositivity from the previous WNV transmission season both in patients with WNV neuroinvasive disease and in asymptomatic persons. A strong negative correlation between IgM antibody levels and AI indicates that in cases with very high IgM levels, determination of IgG avidity may not be necessary. As many patients showed rapid avidity maturation, low IgG avidity is indicative of WNV infection within the previous month.
Databáze: OpenAIRE
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