Harmonic Nanoparticles for Regenerative Research
| Autor: | Luigi Bonacina, Flavio Lorenzo Ronzoni, Darragh Crotty, Adriele Prina-Mello, Jérôme Extermann, Marisa Jaconi, Yuri Volkov, Jean-Pierre Wolf, Thibaud Magouroux, Daniel Ciepielewski, Remi Vernet |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Materials science
genetic structures multi-photon imaging Niobium General Chemical Engineering Nanoparticle Bioengineering Cell Communication 02 engineering and technology ddc:616.07 Regenerative Medicine 01 natural sciences Regenerative medicine General Biochemistry Genetics and Molecular Biology Quantitative Biology::Cell Behavior 010309 optics Nonlinear optical Imaging Three-Dimensional 0103 physical sciences Humans Regeneration Cardiomyocyte differentiation Myocytes Cardiac human embryonic stem cells (ESC) Embryonic Stem Cells Excitation wavelength General Immunology and Microbiology business.industry General Neuroscience air-liquid cultures embryoid bodies (EBs) Second-harmonic generation Cell Differentiation Oxides cardiac contraction 021001 nanoscience & nanotechnology cardiomyocyte differentiation Microscopy Fluorescence Multiphoton Potassium Harmonic Optoelectronics nanoparticles 0210 nano-technology business Excitation Issue 87 |
| Zdroj: | Journal of Visualized Experiments Journal of Visualized Experiments, No 87 (2014) Scopus-Elsevier |
| ISSN: | 1940-087X |
| DOI: | 10.3791/51333 |
| Popis: | In this visualized experiment, protocol details are provided for in vitro labeling of human embryonic stem cells (hESC) with second harmonic generation nanoparticles (HNPs). The latter are a new family of probes recently introduced for labeling biological samples for multi-photon imaging. HNPs are capable of doubling the frequency of excitation light by the nonlinear optical process of second harmonic generation with no restriction on the excitation wavelength. Multi-photon based methodologies for hESC differentiation into cardiac clusters (maintained as long term air-liquid cultures) are presented in detail. In particular, evidence on how to maximize the intense second harmonic (SH) emission of isolated HNPs during 3D monitoring of beating cardiac tissue in 3D is shown. The analysis of the resulting images to retrieve 3D displacement patterns is also detailed. |
| Databáze: | OpenAIRE |
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