MSC/ECM Cellular Complexes Induce Periodontal Tissue Regeneration
| Autor: | Kazuhisa Ouhara, Noriyoshi Mizuno, Manabu Takewaki, Hidemi Kurihara, Shinya Sasaki, Shinji Matsuda, Tsuyoshi Fujita, Katsuhiro Takeda, Souta Motoike, Nao Komatsu, Mikihito Kajiya |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell- and Tissue-Based Therapy Mesenchymal Stem Cell Transplantation Regenerative medicine Ilium Extracellular matrix 03 medical and health sciences Dogs 0302 clinical medicine Animals Bone regeneration General Dentistry Cells Cultured Periodontal Diseases Tissue Engineering Chemistry Regeneration (biology) Mesenchymal stem cell Furcation defect Cell Differentiation Mesenchymal Stem Cells X-Ray Microtomography 030206 dentistry Anatomy Ascorbic acid Extracellular Matrix Cell biology Transplantation Disease Models Animal 030104 developmental biology Guided Tissue Regeneration Periodontal |
| Zdroj: | Journal of Dental Research. 96:984-991 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | Transplantation of mesenchymal stem cells (MSCs), which possess self-renewing properties and multipotency, into a periodontal defect is thought to be a useful option for periodontal tissue regeneration. However, developing more reliable and predictable implantation techniques is still needed. Recently, we generated clumps of an MSC/extracellular matrix (ECM) complex (C-MSC), which consisted of cells and self-produced ECM. C-MSCs can regulate their cellular functions in vitro and can be grafted into a defect site, without any artificial scaffold, to induce bone regeneration. Accordingly, this study aimed to evaluate the effect of C-MSC transplantation on periodontal tissue regeneration in beagle dogs. Seven beagle dogs were employed to generate a premolar class III furcation defect model. MSCs isolated from dog ilium were seeded at a density of 7.0 × 104 cells/well into 24-well plates and cultured in growth medium supplemented with 50 µg/mL ascorbic acid for 4 d. To obtain C-MSCs, confluent cells were scratched using a micropipette tip and were then torn off as a cellular sheet. The sheet was rolled up to make round clumps of cells. C-MSCs were maintained in growth medium or osteoinductive medium (OIM) for 5 or 10 d. The biological properties of C-MSCs were evaluated in vitro, and their periodontal tissue regenerative activity was tested by using a dog class III furcation defect model. Immunofluorescence analysis revealed that type I collagen fabricated the form of C-MSCs. OIM markedly elevated calcium deposition in C-MSCs at day 10, suggesting its osteogenic differentiation capacity. Both C-MSCs and C-MSCs cultured with OIM transplantation without an artificial scaffold into the dog furcation defect induced periodontal tissue regeneration successfully compared with no graft, whereas osteogenic-differentiated C-MSCs led to rapid alveolar bone regeneration. These findings suggested that the use of C-MSCs refined by self-produced ECM may represent a novel predictable periodontal tissue regenerative therapy. |
| Databáze: | OpenAIRE |
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