Stable changes in CD4+ T lymphocyte miRNA expression after exposure to HIV-1
| Autor: | Paola Ronzi, Lucia Lopalco, Massimo Galli, Giacomo Magnani, Andrea Cossarizza, Mariolina Gullì, Linda Bertoncelli, Elisabetta Pilotti, F. Bignami, Nelson Marmiroli, Roberta Ruotolo, Marcello Pinti, Cristina Mussini, Claudio Casoli |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Time Factors Immunology HIV Infections HIV Envelope Protein gp120 Biology Biochemistry Immune system multiply exposed uninfected microRNA Humans Drosha miRNA Innate immune system élite long-term nonprogressors Gene Expression Profiling Cell Biology Hematology T lymphocyte Middle Aged Viral Load Microarray Analysis HIV-1 CD4+ T cells In vitro MicroRNAs Gene Expression Regulation Case-Control Studies biology.protein Female Ex vivo Dicer |
| Zdroj: | Blood. 119:6259-6267 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2011-09-379503 |
| Popis: | MicroRNAs (miRNAs) inhibit HIV-1 expression by either modulating host innate immunity or by directly interfering with viral mRNAs. We evaluated the expression of 377 miRNAs in CD4+ T cells from HIV-1 élite long-term nonprogressors (éLTNPs), naive patients, and multiply exposed uninfected (MEU) patients, and we observed that the éLTNP patients clustered with naive patients, whereas all MEU subjects grouped together. The discriminatory power of miRNAs showed that 21 miRNAs significantly differentiated éLTNP from MEU patients and 23 miRNAs distinguished naive from MEU patients, whereas only 1 miRNA (miR-155) discriminated éLTNP from naive patients. We proposed that miRNA expression may discriminate between HIV-1–infected and –exposed but negative patients. Analysis of miRNAs expression after exposure of healthy CD4+ T cells to gp120 in vitro confirmed our hypothesis that a miRNA profile could be the result not only of a productive infection but also of the exposure to HIV-1 products that leave a signature in immune cells. The comparison of normalized Dicer and Drosha expression in ex vivo and in vitro condition revealed that these enzymes did not affect the change of miRNA profiles, supporting the existence of a Dicer-independent biogenesis pathway. |
| Databáze: | OpenAIRE |
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