The effects of arachidonic acid, PGI2, and 6-keto-PGF1 alpha on cyclic nucleotide concentrations in a ventilated and artificially perfused isolated dog lung
| Autor: | F. D. Lott, Michael R. Littner, G. M. Kazmi |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
medicine.medical_specialty
Physiology Metabolite Indomethacin Prostaglandin 6-Ketoprostaglandin F1 alpha Arachidonic Acids In Vitro Techniques Biochemistry chemistry.chemical_compound Endocrinology Dogs Internal medicine medicine Cyclic AMP Animals Nucleotide Theophylline Cyclic GMP Lung chemistry.chemical_classification Arachidonic Acid Aspirin Tranylcypromine Radioimmunoassay Epoprostenol Perfusion Kinetics medicine.anatomical_structure chemistry Prostaglandins lipids (amino acids peptides and proteins) Arachidonic acid medicine.drug |
| Zdroj: | Prostaglandins, leukotrienes, and medicine. 9(3) |
| ISSN: | 0262-1746 |
| Popis: | In 35 isolated dog lung preparations, the pulmonary circulation of the right lung was completely separated from that of the left so that 1 lung of each preparation could serve as a control. The lungs were ventilated with 14% O 2 , 6% CO 2 and 80% N 2 and the pulmonary circulations were perfused with a dextran-salt-bicarbonate solution containing theophylline. Samples of perfusate were assayed for cyclic AMP and cyclic GMP (radioimmunoassay). Infusions of arachidonic acid (n=6) and PGI 2 (n=4) but not 6-keto-PGF 1α (n=3) into the pulmonary circulation led to increases in cyclic AMP compared to control. Cyclic GMP levels were unchanged by the various infusions. Indomethacin (n=4) and acetylsalicylic acid (n=4) (prostaglandin (PG) synthesis inhibitors), and tranylcypromine (n=4) (a PGI 2 synthetase inhibitor), prevented the cyclic AMP increases from arachidonic acid. This prevention was not the result of interference with the ability of cells to produce or release cyclic AMP since indomethacin (n=3), acetylsalicylic acid (n=3), and tranylcypromine (n=4) did not prevent cyclic AMP increases from PGI 2 . We conclude that infusion of arachidonic acid into the canine lung elevated pulmonary cyclic AMP but not cyclic GMP; that part or all of this increase most likely resulted from conversion of arachidonic acid to products of PG synthesis, particularly PGI 2 ; that infusion of PGI 2 mimicked arachidonic acid in that pulmonary cyclic AMP but not cyclic GMP was elevated, and that 6-keto-PGF 1α , a metabolite of PGI 2 , is unlikely to account for the cyclic AMP increases in this study. |
| Databáze: | OpenAIRE |
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