DHA system mediating aerobic and anaerobic dissimilation of glycerol in Klebsiella pneumoniae NCIB 418
| Autor: | R G Forage, E C Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1982 |
| Předmět: |
Glycerol
Catabolite repression Glycerol dehydratase Dihydroxyacetone Biology Microbiology Enzyme Repression chemistry.chemical_compound Cyclic AMP Anaerobiosis Molecular Biology Phosphotransferases Aerobiosis Klebsiella pneumoniae Phosphotransferases (Alcohol Group Acceptor) chemistry Biochemistry Glycerophosphates Mutation Glycerol dehydrogenase Energy source Anaerobic exercise Research Article |
| Popis: | In Klebsiella pneumoniae NCIB 418, the pathways normally responsible for aerobic growth on glycerol and sn-glycerol 3-phosphate (the glp system) are superrepressed. However, aerobic growth on glycerol can take place by the intervention of the NAD-linked glycerol dehydrogenase and the ATP-dependent dihydroxyacetone kinase of the dha system normally inducible only anaerobically by glycerol or dihydroxyacetone. Conclusive evidence that the dha system is responsible for both aerobic and anaerobic dissimilation of glycerol was provided by a Tn5 insertion mutant lacking dihydroxyacetone kinase. An enzymatically coupled assay specific for this enzyme was devised. Spontaneous reactivation of the glp system was achieved by selection for aerobic growth on sn-glycerol 3-phosphate or on limiting glycerol as the sole carbon and energy source. However, the expression of this system became constitutive. Aerobic operation of the glp system highly represses synthesis of the dha system enzymes by catabolite repression. |
| Databáze: | OpenAIRE |
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