Combined ascorbic acid and T3 produce better healing compared to bone marrow mesenchymal stem cells in an Achilles tendon injury rat model: a proof of concept study
Autor: | Cristina Antonetti Lamorgese Passeri, Clarissa Gissi, Roberta Bernardini, Nicola Maffulli, Silvia Brogini, Francesco Oliva, Rosella Cicconi, Marialucia Gallorini, Milena Fini, Anna Berardi, Lucia Calciano, Francesca Veronesi, Maurizio Mattei |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Male
lcsh:Diseases of the musculoskeletal system Thyroid hormones medicine.medical_treatment Pilot Projects 0302 clinical medicine lcsh:Orthopedic surgery Orthopedics and Sports Medicine Saline Ascorbic acid T3 bone marrow mesenchymal stem cells Tendon Achilles Tendon Animals Ascorbic Acid Cells Cultured Disease Models Animal Drug Therapy Combination Mesenchymal Stem Cell Transplantation Rats Rats Inbred Lew Rupture Triiodothyronine Wound Healing Proof of Concept Study 030222 orthopedics Achilles tendon Cultured Inbred Lew T3 medicine.anatomical_structure Combination Cells Rat model Bone marrow mesenchymal stem cells Andrology 03 medical and health sciences Drug Therapy Achilles tendon injury medicine 030203 arthritis & rheumatology Settore MED/04 - Patologia Generale business.industry Animal T 3 bone marrow mesenchymal stem cells R1 lcsh:RD701-811 Disease Models Surgery lcsh:RC925-935 business Wound healing |
Zdroj: | Journal of Orthopaedic Surgery and Research, Vol 14, Iss 1, Pp 1-10 (2019) |
ISSN: | 1749-799X |
Popis: | Background This pilot study aimed to ascertain whether the local application of ascorbic acid (AA), of T3, and of rat (r) bone marrow mesenchymal stem cells (BMSCs), alone or in all possible combinations, promoted healing after an Achilles tendon injury in a rat model. Methods An Achilles tendon defect was produced in 24 6–8-week-old male inbred Lewis rats. The animals were then randomly divided into eight groups of three rats each. The tendon defect was filled with 50 μL of phosphate-buffered saline (PBS) containing (1) 50 μg/mL AA (AA group), (2) 10−7 M T3 (T3 group), (3) 4 × 106 rBMSCs (rBMSC group), (4) 50 μg/mL AA + 10−7 M T3 (AA + T3 group), (5) 4 × 106 rBMSCs + 50 μg/mL AA (rBMSC + AA group), (6) 4 × 106 rBMSCs + 10−7 M T3 (rBMSC + T3 group), (7) 4 × 106 rBMSCS + 50 μg/mL AA + 10−7 M T3 (rBMSC + AA + T3 group), and (8) PBS only (control group: CTRL). All treatments were administered by local injection immediately after the tendons had been damaged; additionally, AA was injected also on the second and fourth day from the first injection (for groups 1, 4, 5, and 7), and T3 was injected again every day for 4 days (for groups 2, 4, 6, and 7). At 30 days from initial treatment, tendon samples were harvested, and the quality of tendon repair was evaluated using histological and histomorphological analysis. The structure and morphology of the injured Achilles tendons were evaluated using the modified Svensson, Soslowsky, and Cook score, and the collagen type I and III ratio was calculated. Results The group treated with AA combined with T3 displayed the lowest Svensson, Soslowsky, and Cook total score value of all tissue sections at histopathological examination, with fiber structure close to regular orientation, normal-like tendon vasculature, and no cartilage formation. AA + T3 also showed the highest collagen I and the lowest collagen III values compared to all other treatments including the CTRL. Conclusion There are potential benefits using a combination of AA and T3 to accelerate tendon healing. |
Databáze: | OpenAIRE |
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