Combined ascorbic acid and T3 produce better healing compared to bone marrow mesenchymal stem cells in an Achilles tendon injury rat model: a proof of concept study

Autor: Cristina Antonetti Lamorgese Passeri, Clarissa Gissi, Roberta Bernardini, Nicola Maffulli, Silvia Brogini, Francesco Oliva, Rosella Cicconi, Marialucia Gallorini, Milena Fini, Anna Berardi, Lucia Calciano, Francesca Veronesi, Maurizio Mattei
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
lcsh:Diseases of the musculoskeletal system
Thyroid hormones
medicine.medical_treatment
Pilot Projects
0302 clinical medicine
lcsh:Orthopedic surgery
Orthopedics and Sports Medicine
Saline
Ascorbic acid
T3
bone marrow mesenchymal stem cells
Tendon
Achilles Tendon
Animals
Ascorbic Acid
Cells
Cultured
Disease Models
Animal
Drug Therapy
Combination
Mesenchymal Stem Cell Transplantation
Rats
Rats
Inbred Lew
Rupture
Triiodothyronine
Wound Healing
Proof of Concept Study
030222 orthopedics
Achilles tendon
Cultured
Inbred Lew
T3
medicine.anatomical_structure
Combination
Cells
Rat model
Bone marrow mesenchymal stem cells
Andrology
03 medical and health sciences
Drug Therapy
Achilles tendon injury
medicine
030203 arthritis & rheumatology
Settore MED/04 - Patologia Generale
business.industry
Animal
T
3
bone marrow mesenchymal stem cells
R1
lcsh:RD701-811
Disease Models
Surgery
lcsh:RC925-935
business
Wound healing
Zdroj: Journal of Orthopaedic Surgery and Research, Vol 14, Iss 1, Pp 1-10 (2019)
ISSN: 1749-799X
Popis: Background This pilot study aimed to ascertain whether the local application of ascorbic acid (AA), of T3, and of rat (r) bone marrow mesenchymal stem cells (BMSCs), alone or in all possible combinations, promoted healing after an Achilles tendon injury in a rat model. Methods An Achilles tendon defect was produced in 24 6–8-week-old male inbred Lewis rats. The animals were then randomly divided into eight groups of three rats each. The tendon defect was filled with 50 μL of phosphate-buffered saline (PBS) containing (1) 50 μg/mL AA (AA group), (2) 10−7 M T3 (T3 group), (3) 4 × 106 rBMSCs (rBMSC group), (4) 50 μg/mL AA + 10−7 M T3 (AA + T3 group), (5) 4 × 106 rBMSCs + 50 μg/mL AA (rBMSC + AA group), (6) 4 × 106 rBMSCs + 10−7 M T3 (rBMSC + T3 group), (7) 4 × 106 rBMSCS + 50 μg/mL AA + 10−7 M T3 (rBMSC + AA + T3 group), and (8) PBS only (control group: CTRL). All treatments were administered by local injection immediately after the tendons had been damaged; additionally, AA was injected also on the second and fourth day from the first injection (for groups 1, 4, 5, and 7), and T3 was injected again every day for 4 days (for groups 2, 4, 6, and 7). At 30 days from initial treatment, tendon samples were harvested, and the quality of tendon repair was evaluated using histological and histomorphological analysis. The structure and morphology of the injured Achilles tendons were evaluated using the modified Svensson, Soslowsky, and Cook score, and the collagen type I and III ratio was calculated. Results The group treated with AA combined with T3 displayed the lowest Svensson, Soslowsky, and Cook total score value of all tissue sections at histopathological examination, with fiber structure close to regular orientation, normal-like tendon vasculature, and no cartilage formation. AA + T3 also showed the highest collagen I and the lowest collagen III values compared to all other treatments including the CTRL. Conclusion There are potential benefits using a combination of AA and T3 to accelerate tendon healing.
Databáze: OpenAIRE
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