Ion chromatography on a latex-coated silica monolith column
| Autor: | Charles A. Lucy, Karen M. Glenn, Paul R. Haddad |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Anions
geography geography.geographical_feature_category Chromatography Monolithic HPLC column Latex Conductometry Chemistry Silica gel Organic Chemistry Ion chromatography Reproducibility of Results General Medicine engineering.material Chromatography Ion Exchange Silicon Dioxide Biochemistry Analytical Chemistry chemistry.chemical_compound Column chromatography Pulmonary surfactant Coating engineering Nanoparticles Monolith |
| Zdroj: | Journal of Chromatography A. 1155:8-14 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2007.01.098 |
| Popis: | A silica monolith column (Merck Chromolith, 100 mm × 4.6 mm) has been coated with Dionex AS9-SC latex nanoparticles to convert the column into an anion-exchange stationary phase. For comparison purposes, a reversed-phase silica monolith was also converted into an anion-exchange column by coating with the cationic surfactant didodecyldimethylammonium bromide (DDAB). Separations of common inorganic anions were carried out using 7.5 or 5.0 mM 4-hydroxybenzoic acid at pH 7.0 along with suppressed conductivity detection. Direct comparisons were then made between the two columns in terms of selectivity, efficiency and stability. The latex-coated column was on average 50% more efficient than the DDAB-coated column. A 10% decrease in retention times was observed on the DDAB column over 11 h of continuous eluent flow, while the latex coating exhibited |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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