Macrolide-resistant genes of Streptococcus pyogenes isolated from the upper respiratory tract by polymerase chain reaction
| Autor: | Shinji Tamura, Kazuma Yamauchi, Kiyonori Kuki, Noboru Yamanaka, Dewan S. Billal, Jun Mukaigawa, Rinya Sugita, Muneki Hotomi, Keiji Fujihara, Miyoko Endou |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Streptococcus pyogenes Erythromycin Microbial Sensitivity Tests Drug resistance medicine.disease_cause Restriction fragment Microbiology law.invention SmaI Bacterial Proteins law Streptococcal Infections Drug Resistance Bacterial Pulsed-field gel electrophoresis medicine Humans Pharmacology (medical) Serotyping Child Respiratory Tract Infections Polymerase chain reaction biology Infant Clindamycin Middle Aged Virology Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Phenotype Infectious Diseases Child Preschool biology.protein Female Macrolides Polymorphism Restriction Fragment Length medicine.drug |
| Zdroj: | Journal of Infection and Chemotherapy. 10:115-120 |
| ISSN: | 1341-321X |
| DOI: | 10.1007/s10156-004-0302-x |
| Popis: | The growing number of macrolide-resistant strains of Streptococcus pyogenes is an increasing problem worldwide. This study evaluated 300 clinical isolates obtained from the upper respiratory tract. Minimal inhibitory concentrations (MICs) of erythromycin (EM), azithromycin (AZM), and clindamycin (CLDM), serotypes, and macrolide resistance genes of mefA, ermB, and ermTR were determined. The genetic relationship of EM-resistant and susceptible strains were also analyzed by pulsed-field gel electrophoresis (PFGE). Twenty-nine (9.7%) EM-resistant S. pyogenes were identified. Of the 29 strains showing resistance to EM, 22 isolates (7.3%, MIC 3.13-12.5 microg/ml) expressed the mefA gene. The predominant serotypes among the mefA-positive isolates were T12, emm9 or T25, emm75-1. The two isolates (0.1%) that possessed the ermB gene were highly resistant to EM (MIC100 microg/ml). The remaining five strains (1.6%) possessed the ermTR gene (MIC 3.13-100 microg/ml). Restriction fragment polymorphism analyzed by pulsed-field gel electrophoresis (PFGE) by SmaI and ApaI digestions showed several clones among the mefA-positive S. pyogenes. Our findings suggest that the mefA gene is the predominant mechanism for macrolide resistance and that this gene is horizontally transmitted among M phenotype strains of S. pyogenes. Consequently, macrolides would not be the first drug of choice for treatment of tonsillitis and other S. pyogenes-related diseases. Physicians and researchers need to take into consideration the macrolide resistance of some strains of S. pyogenes. |
| Databáze: | OpenAIRE |
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