Control of Bacteria Adhesion by Cell-Wall Engineering
| Autor: | Kenichi Niikura, Reiko Sadamoto, Shin-Ichiro Nishimura, Taichi Ueda, Kenji Monde, Norio Fukuhara |
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| Rok vydání: | 2004 |
| Předmět: |
Gram-positive bacteria
Fluorescence spectrometry Oligosaccharides Biochemistry Bacterial Adhesion Catalysis Cell wall Colloid and Surface Chemistry Fosfomycin Cell Wall Concanavalin A Moiety Surface plasmon resonance Molecular Structure biology Chemistry General Chemistry Adhesion Surface Plasmon Resonance biology.organism_classification Uridine Diphosphate N-Acetylmuramic Acid Anti-Bacterial Agents Lactobacillus biology.protein Bacteria |
| Zdroj: | Journal of the American Chemical Society. 126:3755-3761 |
| ISSN: | 1520-5126 0002-7863 |
| DOI: | 10.1021/ja039391i |
| Popis: | UDP-MurNAc-pentapeptide derivative bacterial cell-wall precursors were synthesized as effective tools for surface display on living bacteria. Lactobacilli were incubated in the ketone-modified precursor-containing medium, and the ketone moiety was displayed on the bacterial surface through cell-wall biosynthesis. Oligomannose was coupled with the ketone moiety on the bacterial surface via a aminooxyl linker, thereby displaying this oligosaccharide on the surface of the bacteria. The increase in the adhesion of the sugar-displaying bacteria onto a concanavalin A-attached film compared to that of native bacteria was confirmed by microscopic observation and surface plasmon resonance measurement. The incorporation of the artificial cell-wall precursors was enhanced when incubated with fosfomycin, an inhibitor of cell-wall precursor biosynthesis. |
| Databáze: | OpenAIRE |
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