Cell Positioning Culture Chip Based on Functional Micro Topological Substrate
| Autor: | Yan Xue, Sa Chenghua, Xie Li, Chen Wei, Fang Ye, Liang Haobin |
|---|---|
| Jazyk: | čínština |
| Rok vydání: | 2020 |
| Předmět: |
Cell localization
chemistry.chemical_classification 0303 health sciences Materials science Biocompatibility General Engineering functional micro topology Substrate (chemistry) Chemical modification TL1-4050 02 engineering and technology Adhesion Polymer 021001 nanoscience & nanotechnology Topology Chip 03 medical and health sciences chemistry cell positioning culture chip Chemical stability 0210 nano-technology micro pattern 030304 developmental biology Motor vehicles. Aeronautics. Astronautics |
| Zdroj: | Xibei Gongye Daxue Xuebao, Vol 38, Iss 1, Pp 139-146 (2020) |
| ISSN: | 2609-7125 1000-2758 |
| Popis: | There are two main categories of cell positioning culture chips:chemical modification of surfaces and physical structure methods. The chemical methods have the advantage of providing a strong binding force, but the instability of chemical materials limits its application. The physical methods have the advantage of good stability for cell positioning, while the nonspecificity of physical structure weakens the ability to regulate cells. Here, we propose a functional micro topological chip. The chip is composed of micro-platform and micro-post array with different adhesion characteristics. At the same time, the chip is prepared by a polymer material with good biocompatibility and high chemical stability; therefore, it has the advantages of strong cell constraint ability and good localization stability. The result of chip performance verification shows that the cell localization rate of the functional micro topological chip is up to 90%, and the chip structure and dimension stability are good. The study provides an important supporting tool for the research of stem cells and their differentiation that need long-term cell culture. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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