In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts
| Autor: | Yoko Oshima-Franco, Patricia Santos Lopes, dos Santos Mg, Leandro Rubem-Mauro, Rocha-Junior Ddos S, Dal-Belo Ca, Vieira Na, Magali Glauzer Silva, V. S. Nazato, da Cruz-Höfling Ma, José Carlos Cogo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Phytochemistry
Myotoxin Pharmaceutical Science Venom complex mixtures Article Analytical Chemistry lcsh:QD241-441 Mice Species Specificity lcsh:Organic chemistry Drug Discovery Botany neurotoxicity Animals Bothrops Physical and Theoretical Chemistry Medicinal plants Dipteryx Methylene Chloride myotoxicity Plants Medicinal biology Traditional medicine Antivenins Plant Extracts Venoms Chemistry Methanol Crotalus Organic Chemistry Dipteryx alata biology.organism_classification Terpenoid Crotalus durissus terrificus Phrenic Nerve Bothrops jararacussu Chemistry (miscellaneous) visual_art Plant Bark visual_art.visual_art_medium Molecular Medicine Bark medicinal plant extract |
| Zdroj: | Molecules; Volume 15; Issue 9; Pages: 5956-5970 Molecules, Vol 15, Iss 9, Pp 5956-5970 (2010) Molecules |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules15095956 |
| Popis: | Extracts from Dipteryx alata bark obtained with different solvents (hexane, dichloromethane, ethyl acetate and methanol) were mixed in vitro with Bothrops jararacussu (Bjssu, 40 μg/mL) and Crotalus durissus terrificus (Cdt, 15 μg/mL) snake venoms, and applied to a mouse phrenic nerve-diaphragm preparation to evaluate the possible neutralization of venom effects. Cdt venom neurotoxic effect was not inhibited by any of the extracts, while the neurotoxic and myotoxic actions of Bjssu venom were decreased by the methanolic extract. This inhibition appears to be augmented by tannins. Dichloromethane bark extract inhibited ~40% of Bjssu venom effects and delayed blockade induced by Cdt. The methodology used to determine which extract was active allows inferring that: (i) phenolic acids and flavonoids contained in the methanolic extract plus tannins were responsible mostly for neutralization of Bjssu effects; (ii) terpenoids from the dichloromethane extract may participate in the anti-Cdt and anti-Bjssu venom effects; (iii) a given extract could not inhibit venoms from different species even if those belong to the same family, so it is improper to generalize a certain plant as antiophidian; (iv) different polarity extracts do not present the same inhibitory capability, thus demonstrating the need for characterizing both venom pharmacology and the phytochemistry of medicinal plant compounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|