Estrogen and progesterone receptors in human papilloma virus-related cervical neoplasia
| Autor: | Clovis Antonio Lopes Pinto, Gilles Landman, Suely Nonogaki, Luisa L. Villa, J S Pereira Sobrinho, G Hamada, Jose C. Prado, F R G Coelho |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Human papillomavirus Pathology medicine.medical_specialty Physiology Immunology Biophysics Uterine Cervical Neoplasms Estrogen receptor Cervix Uteri Biology Cervical intraepithelial neoplasia Polymerase Chain Reaction Biochemistry Progesterone receptor medicine Humans General Pharmacology Toxicology and Pharmaceutics Papillomaviridae lcsh:QH301-705.5 Cervix Cervical cancer lcsh:R5-920 General Neuroscience Carcinoma Papillomavirus Infections Cell Biology General Medicine Middle Aged Uterine Cervical Dysplasia medicine.disease biology.organism_classification Immunohistochemistry Epithelium medicine.anatomical_structure Receptors Estrogen lcsh:Biology (General) DNA Viral Female Receptors Progesterone lcsh:Medicine (General) |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 37, Iss 1, Pp 83-88 (2004) Brazilian Journal of Medical and Biological Research, Volume: 37, Issue: 1, Pages: 83-88, Published: JAN 2004 |
| ISSN: | 0100-879X |
| DOI: | 10.1590/s0100-879x2004000100012 |
| Popis: | Estrogen (ER) and progesterone (PR) receptors in the normal uterine cervix, cervical intraepithelial neoplasia and invasive carcinoma were studied in consecutive samples from Hospital do Câncer, São Paulo, between 1996 and 1997. Tissue was collected by removing a fragment of the tumoral area using a 5-mm diameter biopsy punch, followed by removal of a macroscopically normal area as close as possible from the tumor. Histopathological confirmation was obtained for all specimens analyzed. A total of 24 normal tissues, 17 cases of cervical intraepithelial neoplasia and 7 of invasive carcinomas were studied. The ER/PR ratio was determined by immunohistochemistry using monoclonal antibodies specific for each receptor. Adjacent tissue slides were submitted to generic PCR for human papillomavirus (HPV) DNA detection followed by typing by dot blot hybridization. About half (45.8%) of the tumors were HPV DNA positive while 29.1% of the patients were also HPV positive in their respective normal tissue. ER was negative in the tumoral epithelium of 11 HPV-positive patients (P = 0.04). There was a trend in the ER distribution in normal tissue that was opposite to that from lesions, but it was not statistically significant (P = 0.069). No difference in ER distribution in stromal tissues was observed between HPV-positive and HPV-negative tissues. PR staining was negative in the epithelium of all cases studied. The results obtained from this small number of cases cannot be considered to be conclusive but do suggest that factors related to viral infection affect the expression of these ER/PR cervix receptors. |
| Databáze: | OpenAIRE |
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