Stromal-epithelial interactions modulate the effect of ovarian steroids on goat uterine epithelial cell interleukin-18 release
| Autor: | Xuefeng Qi, Y.P. Jin, A.H. Wang, Y.Y. Qu, X.J. Zhao, Z.C. Nan |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Stromal cell medicine.medical_treatment Blotting Western Enzyme-Linked Immunosorbent Assay Cell Communication Biology Endometrium Cell Line Endocrinology Food Animals Internal medicine medicine Animals RNA Messenger Progesterone Messenger RNA Estradiol Reverse Transcriptase Polymerase Chain Reaction Goats Interleukin-18 Epithelial Cells Epithelium Coculture Techniques Blot stomatognathic diseases Steroid hormone medicine.anatomical_structure Cell culture Animal Science and Zoology Interleukin 18 Female Stromal Cells |
| Zdroj: | Domestic animal endocrinology. 42(4) |
| ISSN: | 1879-0054 |
| Popis: | A primary role of epithelial-stromal interactions in mediating steroid hormone action in the uterus has been established. The present study was undertaken to determine the mode of ovarian steroid action in regulating IL-18 release by goat endometrial epithelial cells (EECs) in the presence and absence of endometrial stromal cells (ESCs). Primary and telomerase-immortalized goat EECs grown alone or cocultured with ESCs were treated with two ovarian steroids, 17β-estradiol (E(2)) and progesterone (P(4)). The IL-18 mRNA and protein expression in EECs were studied by reverse transcript (RT) PCR, ELISA, and Western blot assay. The E(2) and/or P(4) treatment of EECs led to a significant increase in both IL-18 mRNA and protein expression either in the primary or in the immortalized EECs compared with that in EECs without the steroid treatment. However, in the presence of ESCs, IL-18 expression by EECs treated with steroids was significantly decreased compared with cells untreated with E(2) and/or P(4). In addition, significantly high abundance of IL-18 mRNA and protein expression by primary and telomerase-immortalized goat EECs was observed in the presence of ESCs compared with those cells without ESCs. These findings suggest that steroids are important for the control of IL-18 expression in goat EECs. Underlying ESCs are needed to mediate the inhibitory effects of steroids on the IL-18 secretory activity of goat EECs in vitro. The IL-18 abundance expressed by goat EECs in vitro are enhanced by underlying ESCs without the treatment of E(2) and/or P(4). |
| Databáze: | OpenAIRE |
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