A universal approach to gene expression engineering

Autor:	Rahmi Lale, Lisa Tietze, Maxime Fages-Lartaud, Jenny Nesje, Ingerid Onsager, Kerstin Engelhardt, Che Fai Alex Wong, Madina Akan, Niklas Hummel, Jörn Kalinowski, Christian Rückert, Martin Frank Hohmann-Marriott
Rok vydání:	2022
Předmět:	Biomaterials Biomedical Engineering translation Bioengineering transcription Agricultural and Biological Sciences (miscellaneous) artificial promoters 5 ' untranslated region Biotechnology
Zdroj:	Synthetic biology (Oxford, England). 7(1)
ISSN:	2397-7000
Popis:	In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creating artificial expression systems. GeneEE leads to the generation of artificial 5ʹ regulatory sequences (ARES) consisting of promoters and 5ʹ untranslated regions. The ARES lead to the successful recruitment of RNA polymerase, related sigma factors and ribosomal proteins that result in a wide range of expression levels. We also demonstrate that by engaging native transcription regulators, GeneEE can be used to generate inducible promoters. To showcase the universality of the approach, we demonstrate that 200-nucleotide (nt)-long DNA with random composition can be used to generate functional expression systems in six bacterial species, Escherichia coli, Pseudomonas putida, Corynebacterium glutamicum, Thermus thermophilus, Streptomyces albus and Streptomyces lividans, and the eukaryote yeast Saccharomyces cerevisiae.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a14adb01d4066e17ff33568d7478cb2 https://pubmed.ncbi.nlm.nih.gov/36212995 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.