Comparison of three immunoassays for detection of antibodies to Strongyloides stercoralis
| Autor: | Neil W. Anderson, Deborah J. Jespersen, Elitza S. Theel, Stephen D. Merrigan, Marc Roger Couturier, Sarina M. Dornink, Diane M. Klein, Thomas B. Nutman, Joseph Kubofcik |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Clinical Biochemistry Immunology Antibodies Helminth Enzyme-Linked Immunosorbent Assay Immunoglobulin G Strongyloides stercoralis Serology Diagnostic Laboratory Immunology medicine Immunology and Allergy Animals Humans Immunoprecipitation medicine.diagnostic_test biology Clinical Laboratory Techniques Diagnostic Tests Routine biology.organism_classification medicine.disease Molecular biology Strongyloidiasis Immunoassay Strongyloides biology.protein Intestinal nematode Antibody |
| Zdroj: | Clinical and vaccine immunology : CVI. 21(5) |
| ISSN: | 1556-679X |
| Popis: | Due to the limited sensitivities of stool-based microscopy and/or culture techniques forStrongyloides stercoralis, the detection of antibodies to this intestinal nematode is relied upon as a surrogate for determining exposure status or making a diagnosis ofS. stercoralisinfection. Here, we evaluated three immunoassays, including the recently released InBios Strongy Detect IgG enzyme-linked immunosorbent assay (ELISA) (InBios International, Inc., Seattle, WA), the SciMedxStrongyloidesserology microwell ELISA (SciMedx Corporation, Denville, NJ), and the luciferase immunoprecipitation system (LIPS) assay performed at the National Institutes of Health (NIH), for their detection of IgG antibodies toS. stercoralis. A total of 101 retrospective serum samples, previously submitted for routineS. stercoralisantibody detection using the SciMedx assay, were also evaluated by the InBios and LIPS assays. The qualitative results from each assay were compared using a Venn diagram analysis, to the consensus result among the three assays, and each ELISA was also evaluated using the LIPS assay as the reference standard. By Venn diagram analysis, 65% (66/101) of the samples demonstrated perfect agreement by all three assays. Also, the numbers of samples considered positive or negative by a single method were similar. Compared to the consensus result, the overall percent agreement of the InBios, SciMedx, and LIPS assays were comparable at 87.1%, 84.2%, and 89.1%, respectively. Finally, the two ELISAs performed analogously but demonstrated only moderate agreement (kappa coefficient for the two assays, 0.53) with the LIPS assay. Collectively, while the two commercially available ELISAs perform equivalently, neither should be used independently of clinical evaluation to diagnose strongyloidiasis. |
| Databáze: | OpenAIRE |
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