Immunohistochemical analysis of sialic acid and fucose composition in human colorectal adenocarcinoma
| Autor: | A. de Carlos, C. Feijoo-Carnero, Ana Merino-Trigo, Francisco Javier Rodríguez-Berrocal, Butrón M, M. Páez de la Cadena, V. S. Martínez-Zorzano, Julia Fernández-Rodríguez |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pathology
medicine.medical_specialty Ribosome Inactivating Proteins Adenocarcinoma Fucose Aleuria aurantia Statistics Nonparametric Substrate Specificity chemistry.chemical_compound Lectins medicine Humans Antigens Tumor-Associated Carbohydrate Intestinal Mucosa Phytohemagglutinins Neoplasm Staging biology Mucin Lectin General Medicine medicine.disease biology.organism_classification Prognosis Molecular biology Immunohistochemistry N-Acetylneuraminic Acid Sialic acid Staining chemistry biology.protein Neoplasm Recurrence Local Plant Lectins Colorectal Neoplasms Follow-Up Studies |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 21(3) |
| ISSN: | 1010-4283 |
| Popis: | The expression of different sialoglycoconjugates and fucoglycoconjugates in normal mucosa and adenocarcinoma samples from 43 colorectal cancer patients was investigated by using specific lectins and applying a semiquantitative analysis. A pronounced decrease in the intracellular binding of the Maackia amurensis lectin, which recognizes alpha(2,3)-linked sialic acid residues, was found in the tumoral tissue. In contrast, a significant increase in the staining with the Sambucus nigra lectin (SNA I), which binds to alpha(2,6)-linked sialic acid residues, was detected in the epithelial cells as well as in the mucins from tumors. No significant differences in the reactivity with the Aleuria aurantia lectin, which recognizes the sequence Fuc(alpha1,6)GlcNAc, between normal and malignant colorectal tissues were detected. Furthermore, the correlation between lectin-binding profiles and the prognosis of colorectal cancer patients was examined. After an average postoperative follow-up period of 31 months, patients with tumors showing a strong SNA I staining presented a greater probability of disease recurrence. This result suggests that the intensity of staining with SNA I could be a valid parameter for predicting recurrence in colorectal cancer. |
| Databáze: | OpenAIRE |
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