T7 RNA polymerase functions in vitro without clustering

Autor:	Kieran Finan, Peter R. Cook, Joseph P. Torella, Achillefs N. Kapanidis
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	Transcription factories Macromolecular Assemblies Transcription Genetic Biophysics RNA-dependent RNA polymerase lcsh:Medicine RNA polymerase II Biochemistry Biophysics Simulations 03 medical and health sciences chemistry.chemical_compound Viral Proteins Transcription (biology) RNA polymerase Bacteriophage T7 DNA-binding proteins Molecular Cell Biology medicine RNA polymerase I Escherichia coli T7 RNA polymerase RNA synthesis lcsh:Science Biology Polymerase 030304 developmental biology 0303 health sciences Multidisciplinary biology Enzyme Classes Physics 030302 biochemistry & molecular biology lcsh:R Proteins DNA-Directed RNA Polymerases Chromosomes Bacterial Molecular biology Cell biology Enzymes Nucleic acids chemistry biology.protein RNA lcsh:Q medicine.drug Research Article
Zdroj:	PLoS ONE PLoS ONE, Vol 7, Iss 7, p e40207 (2012) PLoS ONE; Vol 7
DOI:	10.1371/journal.pone.0040207
Popis:	Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using 'pulldowns' and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a1388d5e8caa6118ce520afdd610bf4 https://doi.org/10.1371/journal.pone.0040207 Zobrazit plný text záznamu