Quantitative assessment of the kinetics of growth factors release from platelet gel
| Autor: | Chen Yao Su, Ya P. Kuo, Heng Lu Nieh, Thierry Burnouf, Yu H. Tseng |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Blood Platelets Vascular Endothelial Growth Factor A Time Factors medicine.medical_treatment Immunology Biocompatible Materials Diffusion Transforming Growth Factor beta1 Calcium Chloride chemistry.chemical_compound Thrombin Epidermal growth factor medicine Animals Humans Immunology and Allergy Platelet Insulin-Like Growth Factor I Polyacrylamide gel electrophoresis Platelet-Derived Growth Factor Gel electrophoresis Chromatography Epidermal Growth Factor Platelet Count Chemistry Growth factor Plateletpheresis Hematology Blood Protein Electrophoresis Platelet Activation Controlled release Vascular endothelial growth factor Intercellular Signaling Peptides and Proteins Cattle Electrophoresis Polyacrylamide Gel Gels medicine.drug |
| Zdroj: | Transfusion. 48:2414-2420 |
| ISSN: | 1537-2995 0041-1132 |
| Popis: | Background The time course of the release of growth factors from platelet (PLT) gels has not been thoroughly studied and should be elucidated for a better standardization of the clinical use of these products. Study design and methods Release of PLT-derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), and insulin-like growth factor-1 (IGF-1) was determined 5, 60, 120, and 300 minutes after PLT gel formation. Control experiments where PLT gel was removed and, afterward, exogenous thrombin was added, were also performed. Protein profiles of the PLT concentrates and of the releasates were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results Mean PDGF-AB, TGF-beta1, EGF, and VEGF concentration increased to 76, 114, 3.7, and 0.8 ng per mL, respectively, in the presence of the PLT gel, but remained at approximately 28, 30, 0.28, and 0.34 ng per mL, respectively, when the PLT gel was removed after formation. IGF-1 content remained unchanged (approx. 80 ng/mL). SDS-PAGE analysis showed that several PLT proteins disappear during PLT gel formation and that the protein patterns of the releasates were undistinguishable at the different time points. Conclusion There is a gradual and fast release of PDGF-AB, TGF-beta1, EGF, and VEGF from PLT gel for at least 60 to 300 minutes after gel formation, whereas the IGF releasate concentration remains unchanged. This study may provide useful information to improve clinical applications of PLT gels and to design improved blood-derived biomaterials with controlled release of growth factors. |
| Databáze: | OpenAIRE |
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