Intermittent pulsed electromagnetic field stimulation activates the mTOR pathway and stimulates the proliferation of osteoblast-like cells
| Autor: | Kengo Yamamoto, Toshinori Masaoka, Munehiro Date, Yasunobu Sawaji, Hironori Miyamoto, Takahiro Iwaki, Eiichi Fukada |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Physiology
Biophysics Stimulation 02 engineering and technology Bone healing 030218 nuclear medicine & medical imaging Cell Line 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Electromagnetic Fields 0202 electrical engineering electronic engineering information engineering medicine Humans Radiology Nuclear Medicine and imaging Phosphorylation PI3K/AKT/mTOR pathway Cell Proliferation Osteoblasts Chemistry Cell growth TOR Serine-Threonine Kinases 020206 networking & telecommunications Osteoblast General Medicine Alkaline Phosphatase Electric Stimulation Cell biology medicine.anatomical_structure Bromodeoxyuridine Alkaline phosphatase Signal Transduction |
| Zdroj: | Bioelectromagnetics. 40(6) |
| ISSN: | 1521-186X |
| Popis: | Pulsed electromagnetic fields (PEMFs) have been shown to be a noninvasive physical stimulant for bone fracture healing. However, PEMF stimulation requires a relatively long period of time and its mechanism of action has not yet been fully clarified. Recently, the mammalian target of rapamycin (mTOR) pathway has been shown to be involved in bone formation. This study aimed to investigate the effects of PEMFs on osteoblastic MC3T3-E1 cells by examining various cellular responses including changes in the mTOR pathway. Continuous PEMF stimulation induced a transient phosphorylation of the mTOR pathway, whereas intermittent PEMF stimulation (1 cycle of 10 min stimulation followed by 20 min of stimulation pause) revitalized the reduced phosphorylation. Moreover, PEMF stimulation stimulated cell proliferation (bromodeoxyuridine incorporation) rather than differentiation (alkaline phosphatase activity), with a more notable effect in the intermittently stimulated cells. These results suggest that intermittent PEMF stimulation may be effective in promoting bone fracture healing by accelerating cell proliferation, and in shortening stimulation time. Bioelectromagnetics. 2019;40:412-421. © 2019 Bioelectromagnetics Society. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text