Ectopic expression of a pea apyrase enhances root system architecture and drought survival in Arabidopsis and soybean
Autor: | Greg Clark, Roopadarshini Veerappa, Jing Wang, Alexander Siegenthaler, Stanley J. Roux, Robert D. Slocum |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Osmotic shock Physiology Transgene Drought tolerance Arabidopsis Plant Science Genetically modified crops Root hair Biology Plant Roots 01 natural sciences Ectopic Gene Expression 03 medical and health sciences Auxin Plant Proteins chemistry.chemical_classification Dehydration Apyrase fungi Peas food and beverages Plants Genetically Modified biology.organism_classification Cell biology 030104 developmental biology chemistry Plant Stomata Ectopic expression Soybeans 010606 plant biology & botany |
Zdroj: | Plant, Cell & Environment. 42:337-353 |
ISSN: | 1365-3040 0140-7791 |
Popis: | Ectoapyrases (ecto-NTPDases) function to decrease levels of extracellular ATP and ADP in animals and plants. Prior studies showed that ectopic expression of a pea ectoapyrase, psNTP9, enhanced growth in Arabidopsis seedlings and that the overexpression of the two Arabidopsis apyrases most closely related to psNTP9 enhanced auxin transport and growth in Arabidopsis. These results predicted that ectopic expression of psNTP9 could promote a more extensive root system architecture (RSA) in Arabidopsis. We confirmed that transgenic Arabidopsis seedlings had longer primary roots, more lateral roots, and more and longer root hairs than wild-type plants. Because RSA influences water uptake, we tested whether the transgenic plants could tolerate osmotic stress and water deprivation better than wild-type plants, and we confirmed these properties. Transcriptomic analyses revealed gene expression changes in the transgenic plants that helped account for their enhanced RSA and improved drought tolerance. The effects of psNTP9 were not restricted to Arabidopsis, because its expression in soybeans improved the RSA, growth, and seed yield of this crop and supported higher survival in response to drought. Our results indicate that in both Arabidopsis and soybeans, the constitutive expression of psNTP9 results in a more extensive RSA and improved survival in drought stress conditions. |
Databáze: | OpenAIRE |
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