Molecular and physiological aspects of aflatoxin and sterigmatocystin biosynthesis by Aspergillus tamarii and A. ochraceoroseus
| Autor: | M. A. Klich, E. J. Mullaney, Jeffrey W. Cary, C. B. Daly |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Aflatoxin
Sterigmatocystin Aspergillus flavus Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Aflatoxins Gene Expression Regulation Fungal heterocyclic compounds DNA Fungal Mycotoxin Gene Aspergillus biology Nucleic Acid Hybridization food and beverages General Medicine Hydrogen-Ion Concentration biology.organism_classification Culture Media genomic DNA chemistry Biochemistry DNA Probes DNA Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 53:605-609 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | Until recently, only three species (Aspergillus flavus, A. parasiticus and A. nomius) have been widely recognized as producers of aflatoxin. In this study we examine aflatoxin production by two other species, A. tamarii and A. ochraceoroseus, the latter of which also produces sterigmatocystin. Toxin-producing strains of A. tamarii and A. ochraceoroseus were examined morphologically, and toxin production was assayed on different media at different pH levels using thin layer chromatography and a densitometer. Genomic DNA of these two species was probed with known aflatoxin and sterigmatocystin biosynthesis genes from A. flavus, A. parasiticus and A. nidulans. Under the high stringency conditions, A. tamarii DNA hybridized to all four of the A. flavus and A. parasiticus gene probes, indicating strong similarities in the biosynthetic pathway genes of these three species. The A. ochraceoroseus DNA hybridized weakly to the A. flavus and A. parasiticus verB gene probe, and to two of the three A. nidulans probes. These data indicate that, at the DNA level, the aflatoxin and sterigmatocystin biosynthetic pathway genes for A. ochraceoroseus are somewhat different from known pathway genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink