Oviduct epithelial cells-derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos
| Autor: | Seonggyu Bang, Sang Hoon Lee, Jongki Cho, Gyeonghwan Seong, Islam M. Saadeldin, Bereket Molla Tanga, Xun Fang |
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| Rok vydání: | 2021 |
| Předmět: |
Nuclear Transfer Techniques
Swine Parthenogenesis Embryonic Development Oviducts Biology Andrology Embryo Culture Techniques Extracellular Vesicles Pregnancy Gene expression Genetics medicine Animals Humans Blastocyst Embryogenesis Embryo Embryo culture Epithelial Cells Cell Biology medicine.anatomical_structure Apoptosis Culture Media Conditioned embryonic structures Somatic cell nuclear transfer Oviduct Female Developmental Biology |
| Zdroj: | Molecular reproduction and developmentREFERENCES. 89(1) |
| ISSN: | 1098-2795 |
| Popis: | Extracellular vesicles (EVs) carry bioactive cargoes involved in the early preimplantation development. This study investigated the effects of EVs obtained from an oviductal epithelial cell (OEC) conditioned medium on the developmental competence of in parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) porcine embryos. The OEC-EV-treated group showed significant increases in blastocyst formation and hatching rates compared to the control group (40.8% ± 2.2% and 20.1% ± 2.1% vs. 24.9% ± 2.0% and 5.3% ± 1.1%; p < 0.05), respectively. The 7 day OEC-EVs treatment group significantly increased blastocyst formation rate than the 3 day and 0 day-groups (45.0 ± 0.8 vs. 33.0 ± 0.7 and 26.7 ± 0.5; p < 0.05), respectively. SCNT revealed that the OEC-EV increased blastocyst formation rate compared to that of oviductal fluid EVs (OF-EVs) (35.4% ± 1.4% vs. 29.3% ± 1.3%; p < 0.05). Reactive oxygen species levels, apoptosis, and blastocyst lipid content were significantly decreased in the OEC-EVs group compared with the control group. OEC-EV group showed a significantly decreased BAX and increased BCL2, SOD1, POU5F1, SOX2, NANOG, GATA6, PNPLA2, LIPE, and MGLL gene expression than the control group (p < 0.05). In conclusion, OEC-EVs supplementation in embryo culture media improved the quality of porcine embryos, potentially helping porcine-cloned embryonic development possibly through transfer of messenger RNA and proteins to the early embryos. |
| Databáze: | OpenAIRE |
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