Effect of POX genotype and Lip2p overexpression on lactone production and reconsumption by Yarrowia lipolytica using castor oil as substrate
| Autor: | Jean-Marc Nicaud, Adelaide Braga, A. M. Crutz-Le Coq, Isabel Belo, Rémi Dulermo |
|---|---|
| Přispěvatelé: | Centre of Biological Engineering, University of Minho [Braga], MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Project 'BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes [NORTE-07-0124-FEDER-000028], Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER, Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BD/63701/2009], FCT Strategic Project [PEst-OE/EQB/LA0023/2013], Universidade do Minho |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Yarrowia lipolytica
Ciências Agrárias::Biotecnologia Agrária e Alimentar Biotecnologia Agrária e Alimentar [Ciências Agrárias] [SDV]Life Sciences [q-bio] Ricinoleic acid Bioengineering β-Oxidation Applied Microbiology and Biotechnology Biochemistry gamma-Decalactone production ?-Decalactone production chemistry.chemical_compound Hydrolysis gamma décalactone Biotransformation castor oil lipase medicine Bioreactor γ-Decalactone production Lipase Castor oil Science & Technology biology Strain (chemistry) beta oxydation Yarrowia biology.organism_classification huile de ricin 2 oxidation chemistry biology.protein beta-Oxidation yarrowia lipolytica medicine.drug |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Process Biochemistry Process Biochemistry, Elsevier, 2015, 50 (9), pp.1357-1362. ⟨10.1016/j.procbio.2015.05.019⟩ |
| ISSN: | 0032-9592 1359-5113 |
| DOI: | 10.1016/j.procbio.2015.05.019⟩ |
| Popis: | γ-Decalactone production from ricinoleic acid biotransformation by Yarrowia lipolytica has drawn the attention of many authors and, in the last years, molecular and physiological developments were made through the use of engineered strains in novel culture conditions. The purpose of this work was to monitor the performance of modified Y. lipolytica strains in their lipid metabolism specifically at the β-oxidation pathway (MTLY40-2P strain, disrupted in the native genes POX2-5 and expressing an ectopic Aox2p-encoding gene) or at the triglyceride hydrolysis (JMY3010 strain, Lip2p overexpressed) using castor oil as substrate, in a lab-scale bioreactor. Using step-wise fed-batch cultures of MTLY40-2P strain, a γ-decalactone concentration of 7 g L−1 was reached. γ-Decalactone reconsumption was prevented and hydroxylactone production was reduced by Y. lipolytica MTLY40-2P strain. Moreover, substantial increase in the initial rate of aroma production was obtained with strain overexpressing LIP2 gene due to the fast hydrolysis of castor oil. Project ‘BioInd – Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028’ co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER and FCT Strategic Project PEst-OE/EQB/LA0023/2013. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect