Age- and sun exposure-dependent differences in 8-hydroxy-2'-deoxyguanosine and Nε-(carboxymethyl)lysine in human epidermis
| Autor: | Yoriko Yamashita, Shinya Toyokuni, Akira Date, Ayaka Hirao, Shinya Akatsuka, Takashi Yoshii, Akira Kawada, Ryoji Nagai, Tamae Wada |
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| Rok vydání: | 2011 |
| Předmět: |
Nutrition and Dietetics
integumentary system Epidermis (botany) Clinical Biochemistry Lysine Medicine (miscellaneous) 8-Hydroxy-2'-deoxyguanosine Human skin Biology medicine.disease_cause complex mixtures Molecular biology advanced glycation end product UV chemistry.chemical_compound Biochemistry chemistry medicine oxidative stress Advanced glycation end-product Immunohistochemistry Original Article skin aging Immunostaining Oxidative stress |
| Zdroj: | Journal of Clinical Biochemistry and Nutrition |
| ISSN: | 1880-5086 0912-0009 |
| Popis: | Aging and exposure to sunlight are two major factors in the deterioration of skin function. In this study, thirty-six fixed human skin samples from sun-exposed and unexposed areas from young and old individuals were used to evaluate the localization of oxidative stress according to levels and distribution of 8-hydroxy-2'-deoxyguanosine and N(ε)-(carboxymethyl)lysine in samples using immunohistochemistry. In the epidermis of the young, negligible amounts of 8-hydroxy-2'-deoxyguanosine and N(ε)-(carboxymethyl)lysine were detected in unexposed areas, whereas nuclear 8-hydroxy-2'-deoxyguanosine and cytoplasmic N(ε)-(carboxymethyl)lysine were increased in the lower epidermis in sun-exposed areas. In contrast, the aged presented prominent nuclear 8-hydroxy-2'-deoxyguanosine and nuclear N(ε)-(carboxymethyl)lysine in the epidermis of unexposed areas, concomitant with dermal increase in N(ε)-(carboxymethyl)lysine. However, the immunostaining of 8-hydroxy-2'-deoxyguanosine and N(ε)-(carboxymethyl)lysine revealed a decrease in the epidermis of sun-exposed areas in the aged. These results suggest an age-dependent difference in the adaptation and protective mechanisms of the epidermis against sunlight-associated oxidative stress, thus necessitating distinct standards for evaluation in each age group. Further investigation is warranted to elucidate underlying molecular mechanisms. |
| Databáze: | OpenAIRE |
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