Dicer function is required in the metanephric mesenchyme for early kidney development
| Autor: | Jacqueline Ho, Sunder Sims-Lucas, Daniel Bushnell, Jordan A. Kreidberg, Jessica Y. S. Chu, Andrew J. Bodnar |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Ribonuclease III
Mesoderm Physiology Mesenchyme Organogenesis Kidney development Apoptosis Gestational Age Nephron Biology urologic and male genital diseases Kidney Gene Expression Regulation Enzymologic DEAD-box RNA Helicases Mice Proto-Oncogene Proteins microRNA medicine SALL1 Animals Embryonic Stem Cells Mice Knockout Bcl-2-Like Protein 11 urogenital system Gene Expression Regulation Developmental Membrane Proteins Nuclear Proteins Articles Nephrons Molecular biology Cell biology MicroRNAs medicine.anatomical_structure Ureteric bud biology.protein Trans-Activators Ureter Apoptosis Regulatory Proteins Dicer |
| Popis: | MicroRNAs (miRNAs) are small, noncoding regulatory RNAs that act as posttranscriptional repressors by binding to the 3′-untranslated region (3′-UTR) of target genes. They require processing by Dicer, an RNase III enzyme, to become mature regulatory RNAs. Previous work from our laboratory revealed critical roles for miRNAs in nephron progenitors at midgestation (Ho J, Pandey P, Schatton T, Sims-Lucas S, Khalid M, Frank MH, Hartwig S, Kreidberg JA. J Am Soc Nephrol 22: 1053–1063, 2011). To interrogate roles for miRNAs in the early metanephric mesenchyme, which gives rise to nephron progenitors as well as the renal stroma during kidney development, we conditionally ablated Dicer function in this lineage. Despite normal ureteric bud outgrowth and condensation of the metanephric mesenchyme to form nephron progenitors, early loss of miRNAs in the metanephric mesenchyme resulted in severe renal dysgenesis. Nephron progenitors are initially correctly specified in the mutant kidneys, with normal expression of several transcription factors known to be critical in progenitors, including Six2, Pax2, Sall1, and Wt1. However, there is premature loss of the nephron progenitor marker Cited1, marked apoptosis, and increased expression of the proapoptotic protein Bim shortly after the initial inductive events in early kidney development. Subsequently, there is a failure in ureteric bud branching and nephron progenitor differentiation. Taken together, our data demonstrate a previously undetermined requirement for miRNAs during early kidney organogenesis and indicate a crucial role for miRNAs in regulating the survival of this lineage. |
| Databáze: | OpenAIRE |
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