Effect of diabetes on collagen metabolism and hypoxia in human gingival tissue: a stereological, histopathological, and immunohistochemical study
| Autor: | Fikret Gevrek, H. Balci Yuce, Ozkan Karatas, Feyza Tulu, Ahmet Altan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Vascular Endothelial Growth Factor A 0301 basic medicine Pathology medicine.medical_specialty Histology Lysyl hydroxylase Gingiva Matrix metalloproteinase 03 medical and health sciences Oxygen Consumption 0302 clinical medicine Diabetes mellitus medicine Humans Hypoxia Periodontitis 030102 biochemistry & molecular biology biology Procollagen-Lysine 2-Oxoglutarate 5-Dioxygenase business.industry Soft tissue General Medicine Middle Aged Hypoxia (medical) Hypoxia-Inducible Factor 1 alpha Subunit medicine.disease Immunohistochemistry Medical Laboratory Technology Matrix Metalloproteinase 8 Diabetes Mellitus Type 2 Gene Expression Regulation Apoptosis 030220 oncology & carcinogenesis biology.protein Female Collagen medicine.symptom business |
| Zdroj: | Biotechnic & Histochemistry. 94:65-73 |
| ISSN: | 1473-7760 1052-0295 |
| DOI: | 10.1080/10520295.2018.1508745 |
| Popis: | Diabetes mellitus and periodontitis are chronic inflammatory diseases that disrupt soft tissue metabolism. The diseases separately or together increase apoptosis in gingival fibroblast cells and reduce cell renewal. We investigated the effects of diabetes and periodontitis on the composition and structure of gingival connective tissue. We used gingival biopsies from 16 healthy individuals (control group, C), 16 type 2 diabetic patients with chronic periodontitis (diabetes + periodontitis group, D + P) and 16 healthy chronic periodontitis patients (periodontitis group, P). Biopsies were obtained under local anesthesia. Clinical attachment level (CAL), gingival index (GI) and plaque index (PI) were measured prior to gingival biopsies. Fibroblast cells were counted stereologically. Inflammatory cells were counted histomorphometrically. Hypoxia-inducible factor (HIF)-1α, lysyl hydroxylase (PLOD-2), neutrophil collagenase (MMP-8), and vascular endothelial growth factor (VEGF) levels were evaluated immunohistochemically. CAL, GI and PI for the C group were lower than for the other groups (p 0.05). Fibroblast cell counts were lower for the D + P group than for the other groups (p 0.05). Diabetes increased inflammatory cell numbers in the D and D + P groups compared to the C and P groups. MMP-8 levels were higher for the D + P group than for the other groups. VEGF was elevated in both the P and D + P groups compared to the C group, while HIF-1α and PLOD-2 levels were comparable. Diabetes increased tissue destruction and inflammation, and decreased fibroblast cell numbers without affecting collagen crosslinking and HIF-1α levels. |
| Databáze: | OpenAIRE |
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