Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency
| Autor: | B. I. Huckett, G. M. Meyer, S. Ramgareeb, J. M. Richards, S. J. Snyman, N. Haricharan |
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| Rok vydání: | 2006 |
| Předmět: |
Time Factors
DNA Plant Genotype Somatic embryogenesis Embryonic Development Plant Science Biology Transformation Genetic Botany Poaceae fungi Embryogenesis Gene Transfer Techniques food and beverages General Medicine Plants Genetically Modified Saccharum Plant Leaves Transgenesis Horticulture Transformation (genetics) Phenotype Inflorescence Agronomy and Crop Science Explant culture Transformation efficiency |
| Zdroj: | Plant Cell Reports. 25:1016-1023 |
| ISSN: | 1432-203X 0721-7714 |
| DOI: | 10.1007/s00299-006-0148-z |
| Popis: | A rapid in vitro protocol using direct somatic embryogenesis and microprojectile bombardment was investigated to establish the developmental phases most suitable for efficient sugarcane transformation. Immature leaf roll disc explants with and without pre-emergent inflorescence tissue were compared. It was shown that for effective transformation to occur, explants should be cultured for several days to allow initiation of embryo development prior to bombardment. Leaf roll discs with pre-emergent inflorescences showed a higher degree of embryogenic competence than non-flowering explants, and transformation efficiency was higher when explants containing floral initials were bombarded. Despite the occurrence of high numbers of phenotypically negative plants, combining the use of inflorescent leaf roll discs with direct embryogenic regeneration has the potential to improve the speed and efficiency of transgenesis in sugarcane. |
| Databáze: | OpenAIRE |
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