Evaluation of CCL21 role in post-knee injury inflammation and early cartilage degeneration

Autor: Mohan Subburaman, Bouchra Edderkaoui
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Cartilage
Articular
Male
0301 basic medicine
MMP3
Chemokine
Knee Joint
Knees
Gene Expression
Osteoarthritis
Knee Joints
Rats
Sprague-Dawley
Pathogenesis
Chemokine receptor
White Blood Cells
0302 clinical medicine
Skeletal Joints
Animal Cells
Medicine and Health Sciences
Musculoskeletal System
Immune Response
Multidisciplinary
biology
T Cells
medicine.anatomical_structure
Connective Tissue
Medicine
Legs
medicine.symptom
Anatomy
Cellular Types
Cartilage Diseases
Research Article
endocrine system
Histology
Science
Immune Cells
Immunology
Inflammation
Knee Injuries
03 medical and health sciences
Signs and Symptoms
medicine
Genetics
Animals
Skeleton
030203 arthritis & rheumatology
Blood Cells
Chemokine CCL21
business.industry
Cartilage
Correction
Biology and Life Sciences
Cell Biology
medicine.disease
Rats
030104 developmental biology
Biological Tissue
Body Limbs
biology.protein
Clinical Medicine
business
CCL21
Articular Cartilage
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 3, p e0247913 (2021)
ISSN: 1932-6203
Popis: The expression of some chemokines and chemokine receptors is induced during the development of post-traumatic osteoarthritis (PTOA), but their involvement in the pathogenesis of the disease is unclear. The goal of this study was to test whether CCL21 and CXCL13 play a role in PTOA development. For this purpose, we evaluated the expression profiles of the chemokines Ccl21 and Cxcl13, matrix metalloproteinase enzymes Mmp3 and Mmp13, and inflammatory cell markers in response to partial medial meniscectomy and destabilization (MMD). We then assessed the effect of local administration of CCL21 neutralizing antibody on PTOA development and post-knee injury inflammation. The mRNA expression of both Ccl21 and Cxcl13 was induced early post-surgery, but only Ccl21 mRNA levels remained elevated 4 weeks post-surgery in rat MMD-operated knees compared to controls. This suggests that while both CXCL13 and CCL21 are involved in post-surgery inflammation, CCL21 is necessary for development of PTOA. A significant increase in the mRNA levels of Cd4, Cd8 and Cd20 was observed during the first 3 days post-surgery. Significantly, treatment with CCL21 antibody reduced post-surgical inflammation that was accompanied by a reduction in the expression of Mmp3 and Mmp13 and post-MMD cartilage degradation. Our findings are consistent with a role for CCL21 in mediating changes in early inflammation and subsequent cartilage degeneration in response to knee injury. Our results suggest that targeting CCL21 signaling pathways may yield new therapeutic approaches effective in delaying or preventing PTOA development following injury.
Databáze: OpenAIRE
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